Localization by immunogold cytochemistry of the virus-coded 30K protein in plasmodesmata of leaves infected with tobacco mosaic virus

Abstract

The 30K protein of tobacco mosaic virus (TMV) was localized to the plasmodesmata of infected tobacco leaves by immunogold cytochemistry. This protein has been reported to be in the nuclear fraction of TMV-infected protoplasts, but as it has been proposed to function in cell-to-cell transport of virus, probably via the plasmodesmata, intact tissue was investigated with particular attention directed to plasmodesmata and nuclei. Thin sections were made from leaves mechanically inoculated with TMV at different times. Affinity-purified antibodies against a synthetic peptide corresponding to the C-terminal sequence of the 30K protein were used in the incubations, and parallel sections were incubated with antibodies against TMV. The 30K protein label accumulated inside the plasmodesmata, with a maximum 24 hr after inoculation. No specific label was found in the nuclei or at any other site in the cells.