Abstract
Analysis of total liver, liver nuclei, and nuclear fractions obtained from mice given either deionized water (control) or deionized water containing cupric chloride (experimental) revealed the presence of copper in all samples tested including control nuclei and control heterochromatin and euchromatin. Copper content in control euchromatin appear to remain constant at approximately 0.3 μg/mg DNA, while the concentration in control heterochromatin seem to be subject to wider variations. An elevation in total liver copper followed prolonged treatment with metal ( in vivo) and was accompanied by an increase in total nuclear copper. A preferential uptake of copper by heterochromatin was suggested by the majority of metal binding to this fraction; there was no significant metal accumulation in euchromatin. Dialysis equilibrium experiments were carried out at 25°C, pH 6.0 to determine in vitro binding of the two nuclear fractions. Under physiologic ionic strength (154 m m) heterochromatin binds considerable copper, whereas euchromatin fails to bind appreciable amounts. This biological specificity is lost under conditions of low ionic strength (<2 m m NaCl).
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