Abstract
1. In order to identify the molecular interval containing the blue shell gene (O locus), linkage analysis was conducted with three microsatellite markers, (TTA) n , (TG) n and (tg) n , and a SNP in intron 1 of SLCO1C1 (solute carrier organic anion transporter family, member 1C1; A locus) to map the O locus in an F2 resource population of Dongxiang chickens. 2. Linkage analysis based on 98 F2 hens resulted in estimation of the best map order of the O locus with other linked markers as: (TTA) n –(TG) n –A–O–(tg) n . 3. Based on these results, we inferred that the O locus was located between the A and (tg) n loci, that is, Chr1:67,296,991-69,140,571, which is the first genomic sequence interval to be established for the blue eggshell gene.
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