Abstract

The distribution pattern of cellulases in the extracellular and cell associated fractions of Chaetomium erraticum varied depending upon the cultural conditions. The extracellular fractions revealed two forms each of endoglucanase, EG I, EG II and β-glucosidase, β-Glu I, β-Glu II under static and shake conditions. However, the appearance of an additional form of endoglucanase, EG III and β-glucosidase, β-Glu III in static intracellular and cell debris fractions showed a relation of cellulase production with the perithecia development in this fungus. The maximal production of enzymes was observed at 37°C, pH range 5.0–10.0 in the presence of 1–2% carboxy methyl cellulose (CMC) in static cultures (for exoglucanase and β-glucosidase) and shake cultures (for endoglucanase).

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