Local noradrenaline depletion elicits enhanced dopamine activity in the medial prefrontal cortex

Abstract

Event Abstract Back to Event Local noradrenaline depletion elicits enhanced dopamine activity in the medial prefrontal cortex Rita Galosi1*, Attila Toth1, Zoltan Petyko1 and László Lénárd1 1 Hungarian Academy of Sciences , Medical School of University Pecs, Hungary Earlier data showed coupling between dopamine (DA) and noradrenaline (NA) release in the prefrontal cortex. In the present experiments, bilateral neurochemical lesions were carried out to destroy NA terminals in the medial prefrontal cortex (mPFC). In loco injection of 6-hydroxy-DOPA (6-OHDOPA) was applied. In other group, a potent DA uptake inhibitor, GBR12935 was injected before the administration of neurotoxin 6-hydroxy-dopamine (6-OHDA), which acts on both DA and NA terminal. DA and NA contents of the mPFC were determined by HPLC-electrochemical detection post mortem and in vivo. In controls, NA and DA values were 346.21 +/- 36.02 ng/g and 133.29 +/-21.61 ng/g wet weight tissue (average ± s.e.m.), respectively. GBR 12935 treatment alone did not show significant alteration in catecholamine levels. Lesions of NA terminals by 6-OHDOPA depleted the NA level only by 21 % [p<0.05]. 6-OHDOPA lesion caused elevated DA levels by 122 % [p<0.01]. Concentration of NA was reduced by 43 % [p<0.01] do to GBR 12935 + 6-OHDA lesion. The DA levels were elevated in these cases by 195 % [p<0.01]. Extracellular NA and DA concentrations were 4.36 +/- 1.03 and 5.32 +/- 0.7 pg/20μl sample, respectively. NA concentration was no detectable after GBR 12935 + 6-OHDA lesion. Baseline DA concentrations were enhanced [p<0.05]. Local reverse microdialysis of Yohimbine (10μM) α2 antagonist enhanced NA and DA level in the mPFC. Increase in DA concentration was higher following neurochemical lesion of NA terminals [p<0.01]. In summary, decreased NA input enhanced DA activity in the mPFC. This study was supported by ETT 317/2006, NKTH-OTKA K68431 and by the Hungarian Academy of Sciences. Conference: 12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009. Presentation Type: Poster Presentation Topic: Research on the cerebral cortex and related structures Citation: Galosi R, Toth A, Petyko Z and Lénárd L (2009). Local noradrenaline depletion elicits enhanced dopamine activity in the medial prefrontal cortex. Front. Syst. Neurosci. Conference Abstract: 12th Meeting of the Hungarian Neuroscience Society. doi: 10.3389/conf.neuro.01.2009.04.193 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 06 Mar 2009; Published Online: 06 Mar 2009. * Correspondence: Rita Galosi, Hungarian Academy of Sciences , Medical School of University Pecs, Pecs, Hungary, rita.galosi@aok.pte.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Rita Galosi Attila Toth Zoltan Petyko László Lénárd Google Rita Galosi Attila Toth Zoltan Petyko László Lénárd Google Scholar Rita Galosi Attila Toth Zoltan Petyko László Lénárd PubMed Rita Galosi Attila Toth Zoltan Petyko László Lénárd Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.