Abstract
Impaired angiogenesis contributes to delayed wound healing in aging. Hyaluronan (HA) has a close relationship with angiogenesis and wound healing. However, HA content decreases with age. In this study, we used high molecular weight HA (HMW-HA) (1650 kDa), and investigated its effects on wound healing in old rats by local injection. We found that HMW-HA significantly increases proliferation, migration and tube formation in endothelial cells, and protects endothelial cells against apoptosis. Local injection of HMW-HA promotes wound healing by increasing angiogenesis in old rats. HMW-HA increases the phosphorylation of Src, ERK and AKT, leading to increased angiogenesis, suggesting that local injection of HMW-HA promotes wound healing in elderly patients.
Highlights
With aging population increasing worldwide, delayed wound healing in the elderly is a clinical challenge [1]
The results showed the endotoxin amount for all hyaluronan powders was less than 0.01 EU/mg
This data proved that LPS and/or endotoxin are below a guard level and cellular phenomenon should be driven by hyaluronan rather than impurities on the tested sample alone
Summary
With aging population increasing worldwide, delayed wound healing in the elderly is a clinical challenge [1]. Wound repair is a multistep process consisting of hemostasis, inflammatory cell infiltration, tissue regrowth and remodeling [2]. In elderly population, this progression of events is altered, resulting in wounds that heal more slowly compared with wounds in young adults [3]. HA is a polysaccharide belonging to the glycosaminoglycan family of macromolecules This biopolymer consists of repeated disaccharides of (β,1– 4)-D-glucuronic acid-(β,1–3)-N-acetyl-D-glucosamine [6]. It has been reported that native high molecular-weight HA (HMW-HA) is anti-angiogenic [7], whereas degradation products that are of specific size (e.g., 3–10 disaccharide units; o-HA) exhibit pro-angiogenic activity [8, 9]. The HA content decreases in aging, and plays a significant role in wound healing
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
