Local β-glucan source from oyster mushroom (Pleurotus flabellatus) as potential usage in medical applications

Abstract

Event Abstract Back to Event Local β-glucan source from oyster mushroom (Pleurotus flabellatus) as potential usage in medical applications Shaiful A. Mohamad1*, Nur Hafizati Abd Halim1, Choong Yew Keong2, Ahmad Zainuri Mohd Dzomir1, Hafizal Yazid1, Mohd Yusof Hamzah1 and Everina Nuri1 1 Malaysian Nuclear Agency, Malaysia 2 Herbal Medicine Research Centre, Institute for Medical Research, Ministry of Health (Malaysia), Malaysia Background Many applications of mushroom polysaccharides have been reported such as in drug delivery system, skin applications and as immunomodulatory agents. The polysaccharides can be obtained from the fruit body, mycelium, culture broth and spores of mushroom. In this study, oyster mushroom (Pleurotus flabellatus) culture was produced by submerged culture fermentation due to its higher productivity and faster fermentation rate. Methods The biomass obtained from submerged culture fermentation was extracted by using hot water extraction. The polysaccharides were tested using β-glucan kit and also studied using small angle X-ray scattering (SAXS) equipment. Then the polysaccharides were separated using column chromatography before determining the molecular weight using gel permeation chromatography-multi angle laser light scattering (GPC-MALLS). Results Both endopolysaccharide and exopolysaccharide obtained have shown the presence of β-glucan (1,3;1,6). The polysaccharides also showed the presence of high molecular weight (~3×10^6 Da) and low molecular weight components. SAXS results showed that the maximum agglomerate size was ~27 nm and the average size of globular was 2.6 nm. Conclusion The presence of high molecular weight β-glucan showed potential application as immunomodulatory agents. The polysaccharides can be classified as nanobiomaterials. These studies supported potential usage of local compounds in medical applications. Keywords: Pleurotus flabellatus, Immunomodulation, Small angle X ray scattering, β-Glucan, Submerged fermentation Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Poster Presentation Topic: Miscellaneous Citation: Mohamad SA, Abd Halim N, Keong C, Mohd Dzomir A, Yazid H, Hamzah M and Nuri E (2019). Local β-glucan source from oyster mushroom (Pleurotus flabellatus) as potential usage in medical applications. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00112 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 12 Oct 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. Shaiful A Mohamad, Malaysian Nuclear Agency, Kuala Lumpur, Malaysia, shaifulazuar@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Shaiful A Mohamad Nur Hafizati Abd Halim Choong Yew Keong Ahmad Zainuri Mohd Dzomir Hafizal Yazid Mohd Yusof Hamzah Everina Nuri Google Shaiful A Mohamad Nur Hafizati Abd Halim Choong Yew Keong Ahmad Zainuri Mohd Dzomir Hafizal Yazid Mohd Yusof Hamzah Everina Nuri Google Scholar Shaiful A Mohamad Nur Hafizati Abd Halim Choong Yew Keong Ahmad Zainuri Mohd Dzomir Hafizal Yazid Mohd Yusof Hamzah Everina Nuri PubMed Shaiful A Mohamad Nur Hafizati Abd Halim Choong Yew Keong Ahmad Zainuri Mohd Dzomir Hafizal Yazid Mohd Yusof Hamzah Everina Nuri Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.