Local and systemic effects of chronic peritonitis induced by pristane in the absence of galectin-3

Abstract

Event Abstract Back to Event Local and systemic effects of chronic peritonitis induced by pristane in the absence of galectin-3 Camila Brand1*, Felipe L. Oliveira1, Thayse P. Costa1, Filipe E. Abreu1, Radovan Borojevic1, Roger Chammas2 and Márcia C. El-Cheikh1 1 Universidade Federal do Rio de Janeiro, Brazil 2 Universidade de São Paulo, Brazil Recent studies of our group show that galectin-3-/- (gal-3-/-) animals present larger amount of plasma cells in lymphoid organs. Furthermore, in B cell neoplasms, high concentrations of gal-3 provide greater tumor resistance and progression. An important model for induction of plasmacytomas is the injection of pristane, leading to chronic granulomatous inflammation rich in macrophages, plasma cells and peritoneal B cells. Thus, the objectives of this study were to evaluate quantitative and qualitatively peritoneal cavity and lymphoid organs, and analyze formation of oil granulomas due to stimulus of pristane. Animals were intraperitoneally injected with pristane or maintained under physiological conditions. We observed a significant increase of total cell number in peritoneal cavity of injected WT animals, in contrast to reduced cellularity of gal-3-/- animals. Kinetics of cell cycle from peritoneal cavity cells was modified and annexin-V+ cells were increased in gal-3-/- animals. Quantification of peritoneal subpopulations showed an increase in neutrophils, and decreased numbers of both B and T lymphocytes. The spleen presented large amounts of lipid droplets and CD138+ plasma cells abnormally distributed in gal-3-/- animals, forming cell clusters in splenic parenchyma. Tertiary lymphoid organs were formed in both groups, but in gal-3-/- mice less or none lymphoid structures were present in oil granulomas and annexin-V+ cells were increased compared to WT animal. The results obtained allow us to conclude that the kinetics of inflammatory cells occurs differently in the absence of galectin-3, modulating lymphoid and myeloid populations, both in peripheral regions, as peritoneal cavity, spleen and oil granulomas. Keywords: galectin-3, pristane, Peritoneal Cavity, oil granuloma, Lymphoid organs Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune receptors and signaling Citation: Brand C, Oliveira FL, Costa TP, Abreu FE, Borojevic R, Chammas R and El-Cheikh MC (2013). Local and systemic effects of chronic peritonitis induced by pristane in the absence of galectin-3. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00204 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 14 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Mrs. Camila Brand, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil, camilabrandc@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Camila Brand Felipe L Oliveira Thayse P Costa Filipe E Abreu Radovan Borojevic Roger Chammas Márcia C El-Cheikh Google Camila Brand Felipe L Oliveira Thayse P Costa Filipe E Abreu Radovan Borojevic Roger Chammas Márcia C El-Cheikh Google Scholar Camila Brand Felipe L Oliveira Thayse P Costa Filipe E Abreu Radovan Borojevic Roger Chammas Márcia C El-Cheikh PubMed Camila Brand Felipe L Oliveira Thayse P Costa Filipe E Abreu Radovan Borojevic Roger Chammas Márcia C El-Cheikh Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.