Abstract
BackgroundLong noncoding RNAs (lncRNAs) are an important class of pervasive genes involved in a variety of biological functions. They are aberrantly expressed in many types of cancers. In this study, we described lncRNAs profiles in 6 pairs of human renal clear cell carcinoma (RCCC) and the corresponding adjacent nontumorous tissues (NT) by microarray.Methodology/Principal FindingsWith abundant and varied probes accounting 33,045 LncRNAs in our microarray, the number of lncRNAs that expressed at a certain level could be detected is 17157. From the data we found there were thousands of lncRNAs that differentially expressed (≥2 fold-change) in RCCC tissues compared with NT and 916 lncRNAs differentially expressed in five or more of six RCCC samples. Compared with NT, many lncRNAs were significantly up-regulated or down-regulated in RCCC. Our data showed that down-regulated lncRNAs were more common than up-regulated ones. ENST00000456816, X91348, BC029135, NR_024418 were evaluated by qPCR in sixty-three pairs of RCCC and NT samples. The four lncRNAs were aberrantly expressed in RCCC compared with matched histologically normal renal tissues.Conclusions/SignificanceOur study is the first one to determine genome-wide lncRNAs expression patterns in RCCC by microarray. The results displayed that clusters of lncRNAs were aberrantly expressed in RCCC compared with NT samples, which revealed that lncRNAs differentially expressed in tumor tissues and normal tissues may exert a partial or key role in tumor development. Taken together, this study may provide potential targets for future treatment of RCCC and novel insights into cancer biology.
Highlights
Incidence of renal cell carcinoma is the third highest in the urinary system tumors, accounting for approximately 3% of adult malignancies [1]
We presented the Long noncoding RNAs (lncRNAs) expression profiles in six pairs of renal clear cell carcinoma (RCCC) samples compared with nontumorous tissues (NT) samples, several of which were evaluated by qPCR in a total of sixty-three pairs of tissues
Overview of lncRNAs Profiles From the lncRNAs expression profiles, differentially expressed lncRNAs can be found between RCCC and NT samples
Summary
Incidence of renal cell carcinoma is the third highest in the urinary system tumors, accounting for approximately 3% of adult malignancies [1]. RCCC is the most common type and the incidence of which is the highest in adult males in the past six decade. The overwhelming majority transcriptional outputs of the mammalian genome were confirmed to be protein noncoding genes [12]. Those abundant transcriptomes were regarded as ‘‘transcriptional noise’’. Long noncoding RNAs (lncRNAs) are an important class of pervasive genes involved in a variety of biological functions. They are aberrantly expressed in many types of cancers. We described lncRNAs profiles in 6 pairs of human renal clear cell carcinoma (RCCC) and the corresponding adjacent nontumorous tissues (NT) by microarray
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