Abstract
BackgroundLong non-coding RNA (lncRNA) XIST has been implicated in the progression of a variety of tumor diseases. The purpose of this study was to explore the molecular role of lncRNA XIST in human hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC).MethodsThe expression levels of lncRNA XIST, miR-192 and TRIM25 in HBV-related HCC tissues and HepG2.2.15 cells were detected by qRT-PCR. Biological information and luciferin gene reporter assay were performed to detect the interaction among lncRNA XIST, miR-192 and TRIM25. CCk-8 assay, wound healing assay and colony formation assay were conducted to detect the proliferation and migration ability of HepG2.2.15 cells.ResultsqRT-PCR results showed that the expression levels of lncRNA XIST were remarkably increased in HBV-related HCC tissues and HepG2.2.15 cells. In addition, miR-192 was a direct target gene of lncRNA XIST, and the expression of miR-192 and lncRNA XIST were negatively correlated. Moreover, overexpression of miR-192 observably inhibited the proliferation and migration of HCC cells, while overexpression of lncRNA XIST showed an opposite effect. Furthermore, TRIM25 was a direct target of miR-192, and lncRNA XIST could up-regulate the expression of TRIM25 by targeting miR-192.ConclusionLncRNA XIST could up-regulate the expression of TRIM25 by targeting and binding to miR-192, thus accelerating the occurrence and development of HCC.
Highlights
Long non-coding RNA XIST has been implicated in the progression of a variety of tumor diseases
The results showed that the expression of Long non-coding RNA (lncRNA) XIST in hepatocellular carcinoma (HCC) tissues was remarkably up-regulated compared with that in the adjacent tissues group (P < 0.01) (Fig. 1a)
Since lncRNA XIST is regarded as an inactivator for X chromosome, it was speculated that its expression was correlated with patients’ gender
Summary
Long non-coding RNA (lncRNA) XIST has been implicated in the progression of a variety of tumor diseases. Studies have found that lncRNA XIST is abnormally expressed in multiple tumor tissues including non-small-cell lung cancer, glioblastoma, gastric cancer, hepatocellular carcinoma, ovarian cancer, and breast cancer (Li Chang et al 2018b; Ma et al 2017; Song et al 2016; Xiong et al 2017). MiRNAs are a class of endogenous non-coding single-stranded small molecule RNA with a length of about 19–22 nucleotides that involved in various physiological and pathological processes such as cell proliferation, differentiation, and the occurrence and development of cancer (Chiu et al 2015; Thomson and Dinger 2016). Our bioinformatic analysis results showed that miR-192 contained the potential binding sites of lncRNA XIST
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