LncRNA TUG1 Targets miR-222-3p to Take Part in Proliferation and Invasion of Breast Cancer Cells

Abstract

This study aimed to explore LncRNA TUG1 targeted miR-222-3p in the proliferation and invasion of breast cancer (BC) cells. Seventy-six BC patients admitted to our hospital and 62 health check-ups at the same time were selected as the research objects. Among them, the former was seen as the observation group (OG), and the latter was considered as the control group (CG). The clinical significance of LncRNA TUG1 and miR-222-3p in BC was detected. Human BC cell MCF7 and normal human breast epithelial cell MCF-10A were purchased. After cells were transfected with LncRNA TUG1 and miR-222-3p, their proliferation, invasion, and apoptosis were observed, and the relationship between the two was detected. LncRNA TUG1 was highly expressed, while miR-222-3p was low expressed in BC. When the cut-off value (COV) was 2.109, the sensitivity and specificity of detecting LncRNA TUG1 expression in peripheral blood for BC diagnosis were 98.39% and 69.74%, respectively. When the COV was 0.760, the sensitivity and specificity of detecting miR-222-3p expression were 67.11% and 91.94%. The cell proliferation and invasion of the sh-TUG1 group were dramatically higher than those of the other two, while the apoptosis rate was lower (p < 0.05). The cell proliferation and invasion of the si-TUG1r group were lower than those of the TUG1-NC group, while the apoptosis rate was higher (p < 0.05). The cell proliferation and invasion of the miR-222-3p-inhibitor group were dramatically higher than those of the other two, while the apoptosis rate was lower (p < 0.05). The miR-222-3p-mimics group has lower cell proliferation and invasion but higher apoptosis rate than NC group. (p < 0.05) The LncRNA TUG1 and miR-222-3p had the same gene sequence after target gene verification by http://starbase.sysu.edu.cn/. Biological behavior tests showed no difference in cell proliferation, invasion and apoptosis between the sh-TUG1 + miR-222-3p-mimics group and the TUG1-NC group (p > 0.05). TUG1 promotes the proliferation, invasion and inhibit apoptosis of BC cells by targeted regulation of miR-222-3p.