Abstract
BackgroundOral squamous cell carcinoma (OSCC) is a common kind of squamous cell carcinoma of the head and neck, which is a threat to public health. Long noncoding RNAs (lncRNAs) are associated with the development of various diseases, including cancers. LncRNA titin antisense RNA 1 (TTN-AS1) is known as a crucial regulatory factor in several cancers. Nevertheless, the specific functions of TTN-AS1 in OSCC remains obscure.MethodsThe expression of TTN-AS1 in OSCC samples or cells was analyzed through qRT-PCR. Colony formation assay, EdU assay, flow cytometry assay, TUNEL assay and wound healing assay were conducted to estimate the functions of TTN-AS1 in OSCC cells. RIP and luciferase reporter assays were utilized to detect the interaction between TTN-AS1 and miR-411-3p as well as between miR-411-3p and NFAT5.ResultsTTN-AS1 expression was stronger in OSCC cells. Knockdown of TTN-AS1 effectively restrained cell proliferation and migration but had inductive role in apoptosis. Moreover, TTN-AS1 could function as the miR-411-3p sponge in OSCC and miR-411-3p exerted the inhibitory functions on OSCC cell growth. In addition, NFAT5 was proven as the target of miR-411-3p. Rescue assay indicated that overexpressing NFAT5 could reverse the inhibitory function of TTN-AS1 depletion on cell growth.ConclusionlncRNA TTN-AS1 contributed to the progression of OSCC via miR-411-3p/NFAT5 axis.
Highlights
Oral squamous cell carcinoma (OSCC) is a common kind of squamous cell carcinoma of the head and neck, which is a threat to public health
We discovered that titin antisense RNA 1 (TTN-AS1) expression was extremely high in OSCC cells in comparison of normal human squamous epithelial cell (NOK cell) (Fig. 1a)
We found that TTN-AS1 expression in SCC-4 and SCC-9 cells was highest
Summary
Oral squamous cell carcinoma (OSCC) is a common kind of squamous cell carcinoma of the head and neck, which is a threat to public health. LncRNA titin antisense RNA 1 (TTN-AS1) is known as a crucial regulatory factor in several cancers. Oral squamous cell carcinoma (OSCC) is one of the commonest squamous cell carcinomas occurs in the head and neck. It ranks sixth in occurrence and had a high mortality rate [1, 2]. The crucial functions of lncRNAs in the occurrence and development of assorted cancers have been reported through a flow of researches [8, 9]. PVT1 accelerated esophageal carcinoma cell migration and invasion via sponging miR-145 and regulating FSCN1 [10]
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