Abstract
BackgroundT cell receptor gamma locus antisense RNA 1 (TRG-AS1) has been reported to involve in the progression of glioblastoma, however the role and its underlying molecular mechanism in hepatocellular carcinoma (HCC) remain unknown.MethodsQuantitative real-time polymerase chain reaction (RT-qPCR) was applied to detect TRG-AS1 expression in HCC cells. Besides, the proliferation abilities of HCC cells were assessed by colony formation and EdU assays. The migratory and invasive abilities of HCC cells were examined by transwell assays. Imunofluorescence staining (IF) was used to analyze the epithelial–mesenchymal transitions (EMT). The interaction among TRG-AS1, miR-4500 and BTB domain and CNC homolog 1 (BACH1) were proofed by means of RIP and RNA pull down and luciferase reporter assays.ResultsTRG-AS1 was conspicuously overexpressed in HCC cells. TRG-AS1 silencing apparently suppressed HCC cell proliferation, migration, invasion and epithelial-mesenchymal transition (EMT). Mechanism exploration revealed that TRG-AS1 acted as a molecular sponge of miR-4500 to regulate BACH1. MiR-4500 silencing or BACH1 overexpression in BACH1-downregulated cells fully rescued cell proliferation migration, invasion and EMT progress.ConclusionTRG-AS1 regulates HCC progression by targeting miR-4500/BACH1 axis.
Highlights
T cell receptor gamma locus antisense RNA 1 (TRG-AS1) has been reported to involve in the progression of glioblastoma, the role and its underlying molecular mechanism in hepatocellular carcinoma (HCC) remain unknown
TRG‐AS1 plays a tumor‐promoting role in Hepatocellular carcinoma (HCC) To thoroughly investigate the role of long non-coding RNAs (lncRNAs) TRG-AS1 in HCC, we initially conducted Quantitative real-time polymerase chain reaction (RT-qPCR) to evaluate its expression in HCC cells (HCCLM3, MHCC97-H, Huh-7, MHCC97-L and SK-HEP-1) and normal cell THLE-2
RT-qPCR detected that TRG-AS1 expression was effectively cut down in the HCCLM3 and Huh-7 cells transfected with sh-TRG-AS1#1 and sh-TRGAS1#2 compared to sh-negative control (NC) group (Fig. 1b)
Summary
T cell receptor gamma locus antisense RNA 1 (TRG-AS1) has been reported to involve in the progression of glioblastoma, the role and its underlying molecular mechanism in hepatocellular carcinoma (HCC) remain unknown. Hepatocellular carcinoma (HCC) is a type of primary liver cancer, which is regarded as the fifth common cancer worldwide [1]. With the development of medical technology and improvement of health system, more and more advanced therapeutic managements have been applied to treat HCC patients, such as liver transplantation [3], proton beam therapy [4], percutaneous/laparoscopy-assisted radiofrequency ablation [5] and etc. The prognosis of HCC patients remains unfavorable. The underlying molecular mechanism of lncRNAs have been discussed in multiple cancers, including HCC [7, 8]. RGMB-AS1 and CDKN2B-AS1 accelerate tumor metastasis of HCC [11, 12]
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