Abstract
BackgroundTMPO-AS1 is a recently characterized oncogenic lncRNA in ovarian cancer. Its role in other ovary diseases is unknown. This study explored its role in polycystic ovary syndrome (PCOS).MethodsFollicular fluid was extracted from both PCOS patients and controls. The levels of TMPO-AS1 and mature and premature miR-335-5p were analyzed by RT-qPCR. The role of TMPO-AS1 in regulating miR-355-5p maturation in granulosa-like tumor (KGN) cells was analyzed by overexpression experiments. The interaction between TMPO-AS1 and premature miR-335-5p was analyzed by RNA pull-down assay. The subcellular location of TMPO-AS1 in KGN cells was analyzed by nuclear fractionation assay. The role of TMPO-AS1 and miR-335-5p in KGN cell proliferation was analyzed by BrdU assay.ResultsTMPO-AS1 was increased in PCOS, while mature miR-355-5p was decreased in PCOS. TMPO-AS1 overexpression decreased mature miR-355-5p level but increased premature miR-355-5p. TMPO-AS1 was localized in both nucleus and cytoplasm. TMPO-AS1 directly interacted with premature miR-355-5p in KGN cells. TMPO-AS1 increased KGN cell proliferation while miR-355-5p decreased cell proliferation. The co-transfection assay showed that TMPO-AS1 reduced the suppressive effects of miR-355-5p on cell proliferation.ConclusionsTMPO-AS1 might suppress miR-335-5p maturation to participate in PCOS.
Highlights
Polycystic ovary syndrome (PCOS) is a metabolic and endocrine disease due to hormonal disorders [1], which is mainly reflected by the increased androgen level
Long non-coding RNAs and microRNAs lack necessary genetic information for protein synthesis, they participate in human
The results illustrated that TMPO-AS1 (Fig. 1A) and premature miR-355-5p (Fig. 1B) were increased in polycystic ovary syndrome (PCOS) group (p < 0.05)
Summary
Polycystic ovary syndrome (PCOS) is a metabolic and endocrine disease due to hormonal disorders [1], which is mainly reflected by the increased androgen level. It affects premenopausal women [2], leading to prolonged or infrequent menstrual periods. Previous studies have clearly shown that the development of PCOS is at least partially caused by the altered proliferation and survival of granulosa cells [9–11]. Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) lack necessary genetic information for protein synthesis, they participate in human. TMPO-AS1 is a recently characterized oncogenic lncRNA in ovarian cancer. This study explored its role in polycystic ovary syndrome (PCOS)
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