Abstract
Background Long noncoding ribonucleic acids (lncRNAs) were closely related to the development of gastric cancer. This study investigated the effect of SNHG7 on gastric cancer progression and its potential molecular mechanism. Methods SNHG7 and microRNA-485-5p (miR-485-5p) expressions in gastric cancer tissues and cells were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Cell counting kit-8 (CCK-8), wound healing, and transwell experiments were used to detect cell proliferation, migration, and invasion. The dual luciferase reporter assay, RNA immunoprecipitation (RIP) experiment, and Pearson's correlation analysis were used to confirm the relationship between SNHG7 and miR-485-5p. Results SNHG7 expression was increased in human gastric cancer tissues and cells. Knockdown of SNHG7 could notably inhibit the gastric cancer cells proliferation, migration, and invasion. The dual-luciferase reporter assay and RIP experiments proved that miR-485-5p was a direct target of SNHG7. At the same time, further experiments demonstrated that miR-485-5p inhibition reversed the suppression of SNHG7 knockdown on gastric cancer cells proliferation, migration, and invasion. Conclusions SNHG7 knockdown could hamper gastric cancer progression via inhibiting miR-485-5p expression, providing a novel understanding for gastric cancer development.
Highlights
Gastric cancer is one of the most common malignant tumors of the digestive tract [1]. e incidence and death rate of gastric cancer have been decreasing in the past half century, but it is still the second deadliest cancer in the world [2]
Gastric cancer mainly originates from epithelial cells of the gastric mucosa and occurs in the gastric antrum and gastric pylorus [3]. e etiology of gastric cancer is complex, such as genetics, adverse environment, diet, Helicobacter pylori (HP) infection, and others [4]. e occurrence and development of gastric cancer are related to multiple factors and genes
SNHG7 Expression Is Increased in Gastric Cancer
Summary
Gastric cancer is one of the most common malignant tumors of the digestive tract [1]. e incidence and death rate of gastric cancer have been decreasing in the past half century, but it is still the second deadliest cancer in the world [2]. Some studies have shown that dysregulation of lncRNA is closely related to gastric cancer invasion, migration, metastasis, prognosis, and et cetera [11, 12]. XIST inhibition suppressed gastric cancer progression and metastasis through regulating miR-101/ EZH2 [14]. Long noncoding ribonucleic acids (lncRNAs) were closely related to the development of gastric cancer. SNHG7 and microRNA-485-5p (miR-485-5p) expressions in gastric cancer tissues and cells were detected by quantitative real-time polymerase chain reaction (qRT-PCR). SNHG7 expression was increased in human gastric cancer tissues and cells. Knockdown of SNHG7 could notably inhibit the gastric cancer cells proliferation, migration, and invasion. Further experiments demonstrated that miR-485-5p inhibition reversed the suppression of SNHG7 knockdown on gastric cancer cells proliferation, migration, and invasion. SNHG7 knockdown could hamper gastric cancer progression via inhibiting miR-485-5p expression, providing a novel understanding for gastric cancer development
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