LncRNA SNHG5 Modulates Endometrial Cancer Progression via the miR-25-3p/BTG2 Axis.

Shi Li,Riyang Zhao,Lianmei Zhao,Xiaoya Li,Baoen Shan,Sisi Wei,Suli Dai,Cong Zhang,Xiwa Zhao,Yanan Shan

doi:10.1155/2019/7024675

Abstract

Endometrial carcinoma (EC) is one of the most common malignancies of the female genital tract, although the mechanisms of EC initiation and development remain incompletely understood. In this study, we demonstrated that the noncoding RNA SNHG5 can inhibit the proliferation, migration, and invasion of EC cells by suppressing the expression of its putative target miR-25-3p. Overexpression of miR-25-3p significantly promoted the proliferation, migration, and invasion of EC cells. In addition, we showed that miR-25-3p represses the expression of BTG2 by directly binding to the 3′-UTR of BTG2 mRNA. Furthermore, increased miR-25-3p expression and decreased SNHG5 and BTG2 expression were observed in EC tissues, and the expression of SNHG5 was negatively correlated to that of miR-25-3p but positively correlated to that of BTG2. In summary, for the first time, we report that the SNHG5/miR-25-3p/BTG2 axis plays an important role in EC progression and is of great potential clinical significance for EC diagnosis and therapy.

Highlights

Endometrial carcinoma (EC) is one of the most common female malignancies that threaten health and life [1]
LncRNAs have gained a great deal of attention due to their association with cancer development. long ncRNAs (lncRNAs) are larger than 200 nucleotides and lack an openreading frame to be translated into protein [6, 7]. ese transcripts can participate in the regulation of specific genes through different molecular mechanisms in almost every stage of the expression process [8,9,10,11,12,13,14]
To investigate the clinical significance of SNHG5 in EC, we analyzed its expression using the public tumor sequencing databases GEPIA and TIMER. e results of the analysis showed that SNHG5 exhibited significantly lower expression in EC tumor tissues compared to normal tissues (Figure 1(a))

Summary

Introduction

Endometrial carcinoma (EC) is one of the most common female malignancies that threaten health and life [1]. Based on histopathological and endocrine factors, EC is traditionally classified into two different types: estrogen-dependent type I and estrogen-independent type II [2, 3]. The incidence of EC is increasing, highlighting the importance of investigating the underlying mechanisms of EC initiation. E majority of the human genome comprises noncoding DNA, and their products noncoding RNAs, such as long ncRNAs (lncRNAs) and microRNAs (miRNAs), have been shown to be important regulators in biological processes [5]. Ese transcripts can participate in the regulation of specific genes through different molecular mechanisms in almost every stage of the expression process [8,9,10,11,12,13,14]. An emerging hypothesis that lncRNAs work as competing endogenous RNAs (ceRNAs) has been supported by numerous studies

Methods

Results

Conclusion