Abstract
BackgroundIt has been reported that the lncRNA SNHG16 has significantly increased expression in pancreatic adenocarcinoma (PC). However, the functions and mechanisms of SNHG16 are not clear. The aim of this study was to explore the effects of SNHG16 on PC.MethodsqRT-PCR analysis was applied to detect the expression levels of SNHG16, miR-302b-3p and SLC2A4 in PC tissues and cells. CCK8 and EdU assays were used to evaluate the proliferation of PC cells. Transwell assays were used to assess PC cell migration and invasion. Apoptosis was evaluated by flow cytometry, and the expression of apoptosis-related proteins (including Bax, Bcl-2, cleaved caspase-3 and cleaved caspase-9) was tested by western blotting. The interactions between miR-302b-3p and SNHG16 or miR-302b-3p and the 3’UTR of SLC2A4 mRNA were clarified by a dual luciferase reporter assay and RNA immunoprecipitation.ResultsSNHG16 expression was significantly elevated in PC tissues and cell lines and was associated with poor prognosis of PC patients. Knockdown of SNHG16 reduced PC cell proliferation, migration and invasion. SNHG16 acted as a sponge to regulate miR-302b-3p expression in PC cells. In addition, miR-302b-3p targeted SLC2A4 directly.ConclusionsSNHG16 promoted the progression of PC via the miR-302b-3p/SLC2A4 axis and was expected to be a potential target for the early diagnosis and treatment of PC.
Highlights
Pancreatic adenocarcinoma (PC) is one of the most severe gastrointestinal malignancies and is the fourth most common cause of cancer-related death [1]
LncRNA Small nucleolar RNA host gene 16 (SNHG16) is elevated in pancreatic adenocarcinoma (PC) tissues and cell lines In this study, the expression level of SNHG16 in human PC tissues was first evaluated by qRT-PCR
SNHG16 affects PC cell proliferation and apoptosis To explore the roles of SNHG16 in the PC process, we established BxPC3 and Panc-1 cells with stable silencing of SNHG16, which were named sh-SNHG16 cells (Fig. 2a)
Summary
Pancreatic adenocarcinoma (PC) is one of the most severe gastrointestinal malignancies and is the fourth most common cause of cancer-related death [1]. It has been proven that only 2% of the genome sequence is capable of coding proteins, whereas more than 95% of transcripts are identified as noncoding RNAs [4]. Long noncoding RNAs (lncRNAs) are a class of noncoding RNAs that have no protein-coding ability and are longer than 200 nt. Emerging evidence demonstrates that lncRNAs are involved in numerous malignancies, including PC [5,6,7]. It has been reported that the lncRNA SNHG16 has significantly increased expression in pancreatic adenocarcinoma (PC). The aim of this study was to explore the effects of SNHG16 on PC
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
