Abstract
Cervical cancer (CC) is one of the most common malignancies in females, with high prevalence and mortality globally. Despite advances in diagnosis and therapeutic strategies developed in recent years, CC is still a major health burden worldwide. The molecular mechanisms underlying the development of CC need to be understood. In this study, we aimed to demonstrate the role of lncRNA SNHG15 in CC progression. Using qRT-PCR, we determined that lncRNA SNHG15 is highly expressed in CC tumor tissues and cells. lncRNA SNHG15 knockdown also reduces the tumorigenic properties of CC in vitro, as determined using the MTT, EdU, flow cytometry, and transwell assays. Using bioinformatics analysis, RNA pull-down, ChIP, and luciferase reporter assays, we verified the molecular mechanisms of lncRNA SNHG15 in CC progression and found that lncRNA SNHG15 expression in CC cells is transcriptionally regulated by SOX12; moreover, lncRNA SNHG15 promotes CC progression via the miR-4735-3p/HIF1a axis. This study can provide a potential target for CC diagnosis or therapeutic strategies in the future.
Highlights
Cervical cancer (CC) ranks next to breast cancer as the most common malignancy and is the third leading cause of cancer-related deaths in females globally [1]
Emerging evidence has elucidated the crucial role of Long noncoding RNAs (lncRNAs) in various diseases, including cancers. lncRNA NBR2 suppresses tumorigenesis in hepatocellular cancer via modulating autophagy level [6]. lncRNA GATA3-AS1 regulates triple-negative breast cancer by facilitating tumorigenesis and immune escape phenomena [7]. lncRNA HOTAIR facilitates exosome secretion in hepatocellular cancer by regulating RAB35 and SNAP23 [8]. lncRNA ASB16-AS1 plays an oncogenic role in renal cell carcinoma progression by acting as a ceRNA for miR-185-5p/miR-214-3p [9]
We investigated the molecular mechanisms of lncRNA SNHG15 in CC cells by conducting bioinformatics analysis, RNA pull-down, Chromatin Immunoprecipitation (ChIP), and luciferase reporter assay
Summary
Cervical cancer (CC) ranks next to breast cancer as the most common malignancy and is the third leading cause of cancer-related deaths in females globally [1]. Emerging evidence has elucidated the crucial role of lncRNAs in various diseases, including cancers. LncRNA NBR2 suppresses tumorigenesis in hepatocellular cancer via modulating autophagy level [6]. LncRNA GATA3-AS1 regulates triple-negative breast cancer by facilitating tumorigenesis and immune escape phenomena [7]. LncRNA HOTAIR facilitates exosome secretion in hepatocellular cancer by regulating RAB35 and SNAP23 [8]. LncRNA ASB16-AS1 plays an oncogenic role in renal cell carcinoma progression by acting as a ceRNA for miR-185-5p/miR-214-3p [9]. The function of lncRNA SNHG15 in various cancers has been investigated, including breast cancer, colorectal cancer, gastric cancer, hepatocellular cancer, and lung cancer [12,13,14,15,16].
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