Abstract
The incidence and death rate of colorectal cancer (CRC) is very high, which brings great need to understand the early molecular events of CRC. These studies demonstrate that long noncoding RNA (lncRNA) plays an important role in the occurrence and development of human cancer. Small nucleolar RNA host gene 15 (SNHG15) was recently identified as a cancer‐related lncRNA. In this study, we aimed to evaluate the function and mechanism of SNHG15 in CRC. The expression of SNHG15 was detected by quantitative RT‐PCR (qRT‐PCR) in CRC tissues and matched noncancerous tissues (NCTs). CCK‐8 assay, colony formation assay, flow cytometric analysis, and nude mouse xenograft mode were used to examine the tumor‐promoting function of SNHG15 in vitro and in vivo. The binding relationship between SNHG15, miR‐338‐3p and the target genes of miR‐338‐3p were screened and identified by databases, qRT‐PCR, dual luciferase reporter assay and western blot. Our results showed that SNHG15 was up‐regulated in CRC tissues compared with paired NCTs (P < 0.0001). High level of SNHG15 expression predicted poor prognosis of CRC (P = 0.0051). SNHG15 overexpression could promote cell proliferation and inhibit cell apoptosis. Animal experiments showed that up‐regulation of SNHG15 promoted tumor growth in vivo. The results of mechanism experiments showed that SNHG15 could bind to miR‐338‐3p and block its inhibition on the expression and activity of FOS or RAB14. In conclusion SNHG15 promotes cell proliferation through SNHG15/miR‐338‐3p/FOS‐RAB14 axis in CRC.
Highlights
We found that Small nucleolar RNA host gene 15 (SNHG15) was significantly up‐ regulated in Colorectal cancer (CRC) tissues and associated with poor survival
These data suggest that SNHG15 functions as an oncogene in CRC, and can be used as a potential prognostic biomarker and therapeutic target for CRC
Our previous studies revealed a variety of CRC related long noncoding RNA (lncRNA), which are involved in cancer promoting.[14,15,16,17]
Summary
Hospital Management Centre of Wuxi, Grant/Award Number: YGZXZ1401; Medical Innovation Team Program of Wuxi, Grant/Award Number: CXTP003; Fundamental Research Funds for the Central Universities, Grant/Award Number: JUSRP51710A and NOJUSRP51619B; National Natural Science Foundation of China, Grant/Award Number: 81672328, 81802469, 81772636 and 81802462; Medical Key Professionals Program of Jiangsu Province, Grant/Award Number: AF052141; National First‐class Discipline Program of Food Science and Technology, Grant/Award Number: JUFSTR20180101; Natural Science Foundation of Jiangsu Province, Grant/Award Number: BK20150004, BK20151108 and BK20180618; Project of the Wuxi Health and Family Planning Commission, Grant/ Award Number: Z201806
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