Abstract
Emerging studies indicate important roles for non-coding RNAs (ncRNAs) as essential regulators in myogenesis, but relatively less is known about their function. In our previous study, we found that lncRNA-Six1 can regulate Six1 in cis to participate in myogenesis. Here, we studied a microRNA (miRNA) that is specifically expressed in chickens (miR-1611). Interestingly, miR-1611 was found to contain potential binding sites for both lncRNA-Six1 and Six1, and it can interact with lncRNA-Six1 to regulate Six1 expression. Overexpression of miR-1611 represses the proliferation and differentiation of myoblasts. Moreover, miR-1611 is highly expressed in slow-twitch fibers, and it drives the transformation of fast-twitch muscle fibers to slow-twitch muscle fibers. Together, these data demonstrate that miR-1611 can mediate the regulation of Six1 by lncRNA-Six1, thereby affecting proliferation and differentiation of myoblasts and transformation of muscle fiber types.
Highlights
Since the implementation of the Human Genome Project, scientists have been trying to explore the biological significances of genomic nucleic acid sequences
The results showed that miR-1611 could perfectly bind with and target to interact with both lncRNA-Six1 position 1 and the 30 untranslated region (UTR) of Six1
The present study reveals a role of miR-1611 in the proliferation and differentiation of myoblasts, as well as the transformation of skeletal muscle fiber types
Summary
Since the implementation of the Human Genome Project, scientists have been trying to explore the biological significances of genomic nucleic acid sequences. Recent studies have demonstrated that lncRNAs play crucial roles in wide range of biological processes, and they can regulate gene expression via epigenetic alterations, as well as transcriptional and posttranscriptional processing [16,17,18,19]. Recent studies have found that lncRNAs can act as endogenous competitive RNAs that interact with miRNAs and participate in the regulation of target gene expression [24,25,26,27]. MiR-1611 functions to regulate myoblast proliferation and differentiation, and is involved in the formation of skeletal muscle fibers, which supports the idea of its regulation of lncRNA-Sx1 and Six1 This result further complements the understanding of molecular regulation of lncRNA in chicken skeletal muscle development, and provides theoretical support for lncRNA being responsible for regulating protein-encoding genes through multiple pathways
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