Abstract
BackgroundAccumulating evidence indicates that long non-coding RNAs (lncRNAs) play a crucial role in tumorigenesis. Here, we report a novel lncRNA, RP11-436H11.5, that regulates renal cell carcinoma (RCC) cell proliferation and invasion by sponging miR-335-5p.MethodsExpression of lncRNA RP11-436H11.5 was determined by a qRT-PCR assay in RCC tissues. RCC cell proliferation and invasion were measured by a cell proliferation assay and a transwell invasion assay. Expression of BCL-W was detected by a western blot assay. Interactions between lncRNA RP11-436H11.5 and miR-335-5p were measured by a luciferase reporter assay and a RNA-pull down assay. In vivo experiments were used to detect tumor formation.ResultsIn this study, the qRT-PCR results illustrated that lncRNA RP11-436H11.5 was more highly expressed in RCC tissues than in adjacent normal renal tissues. The results of survival analysis indicated that patients in the high lncRNA RP11-436H11.5 group presented significantly worse outcomes compared with those in the low lncRNA RP11-436H11.5 group. Downregulation of lncRNA RP11-436H11.5 suppressed RCC cell proliferation and invasion in vitro and in vivo. Luciferase reporter assay results demonstrated that lncRNA RP11-436H11.5 enhanced BCL-W expression by regulating miR-335-5p expression. LncRNA RP11-436H11.5 could function as a miR-335-5p decoy to derepress expression of BCL-W.ConclusionsLncRNA RP11-436H11.5 could function as a competing endogenous RNA to promote RCC cell proliferation and invasion, which might serve as a therapeutic application to suppress RCC progression.
Highlights
Accumulating evidence indicates that long non-coding RNAs play a crucial role in tumorigenesis
Long non-coding RNAs (LncRNAs) RP11-436H11.5 expression is upregulated in renal cell carcinoma (RCC) tissues Recently, many studies have indicated that long non-coding RNAs (lncRNAs) can regulate gene expression by complementary binding with miRNAs [9, 20]
The results showed that lncRNA RP11-436H11.5 was the most appropriate lncRNA as it was more highly expressed in RCC tissues than in adjacent normal renal tissues (Fig. 1a)
Summary
Accumulating evidence indicates that long non-coding RNAs (lncRNAs) play a crucial role in tumorigenesis. Drug resistance can occur in late stage patients, Long non-coding RNAs (lncRNAs) are a set of nonprotein coding transcripts longer than 200 nucleotides [7]. They have been shown to have various functions including post-transcriptional regulation, chromatin modification and other biological processes [8, 9]. Some lncRNAs have been shown to play crucial roles in different kinds of cancer cells, including breast [10], colorectal [11], gastric [12] and renal [13] cancer cells
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