Abstract
A growing number of studies have shown that long-chain non-coding RNA (lncRNA) plays an important role in the progression of non-small cell lung cancer (NSCLC). To explore the role and potential molecular mechanism of lncRNA PSMA3-AS1 in promoting the proliferation, migration and invasion of NSCLC. The expression of PSMA3-AS1, miR-17-5p and PD-L1 in a human bronchial epithelial cell line, BEAS-2B, and NSCLC cell lines, H226 and A549, were detected with quantitative real-time polymerase chain reaction (qRT-PCR) or western blot. The PSMA3-AS1 shRNA transfection was used to reduce the expression of PSMA3-AS1. Double fluorescent enzyme reporting was used to detect the relationship between PSMA3-AS1, miR-17-5p and PD-L1. Cell Counting Kit-8 (CCK-8), wound-healing and transwell assays, as well as western blot, were used to detect the expression of proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT)-related proteins in lung cancer cells. The expression of PSMA3-AS1 in NSCLC cells was significantly higher than in human bronchial epithelial cells. The PSMA3-AS1 knockdown significantly reduced the proliferation, migration and invasion of lung cancer cells. In addition, double fluorescent enzyme results showed that PSMA3-AS1 could competitively bind miR-17-5p to PD-L1. The expression of miR-17-5p is low in lung cancer cells, while the expression of PD-L1 in them is high. Overexpression of PD-L1 reversed the inhibitory effect of PSMA3-AS1 knockdown on the proliferation, migration and invasion of lung cancer cells. Generally speaking, PSMA3-AS1 is highly expressed in NSCLC. The PSMA3-AS1 can promote the proliferation, migration and invasion of NSCLC cells by regulating miR-17-5p/PD-L1.
Highlights
A growing number of studies have shown that long-chain non-coding RNA plays an important role in the progression of non-small cell lung cancer (NSCLC)
Double fluorescent enzyme results showed that PSMA3-AS1 could competitively bind miR-17-5p to PD-L1
Cheng G, Li Y, Liu Z, Song X. long-chain non-coding RNA (lncRNA) PSMA3-AS1 promotes the progression of non-small cell lung cancer through targeting miR-17-5p/PD-L1
Summary
A growing number of studies have shown that long-chain non-coding RNA (lncRNA) plays an important role in the progression of non-small cell lung cancer (NSCLC). Long chain non-coding RNA (lncRNA) is a non-coding RNA with a length of more than 200 bp that does not encode a protein It can regulate gene expression through complex molecular mechanisms at many cellular levels, but the specifics of the mechanisms are not clear.[3] Recent studies have shown that lncRNA, as a regulatory factor, participates in almost all cellular processes and plays a role in promoting or inhibiting cancer.[4,5] For example, high levels of expression of lncRNA PART1 was found to significantly accelerate the occurrence of NSCLC cancer.[6] The lncRNA BRCAT54 overexpression promoted proliferation and migration, and activated apoptosis in NSCLC cells by binding RPS9.7 The lncRNA PSMA3-AS1 is located on chromosome 14 at position 14q23.1 and is overexpressed in esophageal cancer and glioma.[8,9] the study of PSMA3-AS1 in NSCLC is currently less pressing
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