Abstract
The main cause of steroid-induced necrosis of femoral head (SNFH) is excessive glucocorticoid (GC) intake. The aim of this article was to investigate the role of lncRNA NEAT1 as a molecular sponge to adsorb miR-23b-3p and regulate CYP1A2 in SNFH. Fluorescence in situ hybridization was used to localize lncRNA NEAT1. Human bone marrow mesenchymal stem cells (hBMSCs) were collected from patients with SNFH. The expression of lncRNA NEAT1, miR-23b-3p and CYP1A2 in hBMSCs were intervened. Compared to the control group, the lncRNA NEAT1 and CYP1A2 expression in the SNFH group was increased, while miR-23b-3p expression was decreased. GCs could inhibit the osteogenic differentiation of hBMSCs and upregulate the expression of lncRNA NEAT1. Knockdown of lncRNA NEAT1 could promote the proliferation and osteogenic differentiation of hBMSCs in the SNFH group. Overexpression of miR-23b-3p could partially counteract the effect of lncRNA NEAT1 on hBMSCs. CYP1A2 was confirmed to be a target of miR-23b-3p. Overexpression of CYP1A2 could partially rescue the effect of miR-23b-3p overexpression on hBMSCs. In conclusion, lncRNA NEAT1 as a ceRNA can adsorb miR-23b-3p and promote the expression of CYP1A2, which then inhibits the osteogenic differentiation of hBMSCs and promotes the progress of SNFH.
Highlights
Osteonecrosis of the femoral head (ONFH) is known as avascular necrosis of the femoral head
The results showed that compared to the control group, the Alkaline phosphatase (ALP) staining of Human bone marrow mesenchymal stem cells (hBMSCs) induced by dexamethasone was lighter (Figure 1a), and the calcium nodules stained by Alizarin red staining (ARS) were fewer (Figure 1b), indicating the osteogenic differentiation ability of hBMSCs in the dexamethasoneinduced group was weaker than that of the control group
The diagnostic value of Long non-coding RNAs (lncRNAs) NEAT1 in steroid-induced necrosis of femoral head (SNFH) was analyzed by the Receiver operating characteristic (ROC) curve, and the result showed that the value of area under curve was above 0.85
Summary
Osteonecrosis of the femoral head (ONFH) is known as avascular necrosis of the femoral head. It refers to the structural changes caused by the damage to the blood supply of the femoral head. It is one of the common diseases of lower limb bone disability in young adults [1]. According to the different causes of the disease, ONFH is divided into traumatic and non-traumatic femoral head necrosis. Most non-traumatic femoral head necrosis is caused by glucocorticoids (GCs) [2]. People’s understanding of steroid-induced necrosis of femoral head (SNFH) has increased. Apoptosis is autonomous and programmed cell death, which plays an important role in the progress of many diseases [5]
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