Abstract

 
 
 
 Purpose: Breast cancer has over the years been one of major acute carcinomas in women. This study investigated the fundamental mechanistic functions of the lncRNA-NEAT1/miR-148a-3p/Wnt/β-catenin axis in moderating cell viability, apoptosis and autophagy in Breast Cancer (BC).
 Methods: RT-qPCR measured expression of lncRNA NEAT1 and microRNA-148a-3p in human cell lines for Breast Cancer. Cell transfection upregulated or silenced the genes with CCK-8, western blot and FCM apoptosis assays determining the cellular growth, proliferation and protein expression related to autophagy biomarkers. Furthermore, luciferase assay analyzed the luciferase activity of lncRNA- NEAT1 and microRNA-148a-3p
 Results: The outcomes indicated that LncRNA-NEAT1 was upregulated in BC cell lines and promoted cell viability, autophagy and inhibited Apoptosis in BC cells. However, lncRNA-NEAT1 knockdown inhibited cell viability, autophagy and enhanced apoptosis. In addition, lncRNA-NEAT1 directly targeted microRNA-148a-3p. And, it was found that microRNA-148a-3p overturns the cellular viability, autophagy and inhibitory effects on Apoptosis imposed by lncRNA-NEAT1 overexpression. Lastly, overexpressed lncRNA-NEAT1 activated the Wnt/β-catenin regulatory network through sponging microRNA-148a-3p in BC cell lines.
 Conclusion: The present study showcased that lncRNA-NEAT1 could enhance tumor development in breast cancer via playing the role of molecular sponge to microRNA-148a-3p, and eventually hyper invigorating the Wnt/β-catenin regulatory network.
 
 
 
Highlights
Breast Cancer (BC) is one of the malignant illness prevalent among women across the globe and is very fatal with an approximated 2.4 million fresh incidences and 523 000 fatalities registered in 2015 [1]
Tropical Journal of Pharmaceutical Research is indexed by Science Citation Index (SciSearch), Scopus, International Pharmaceutical Abstract, Chemical Abstracts, Embase, Index Copernicus, EBSCO, African Index Medicus, JournalSeek, Journal Citation Reports/Science Edition, Directory of Open Access Journals (DOAJ), African Journal Online, Bioline International, Open-J-Gate and Pharmacy Abstracts
We investigate the role of long-non coding RNAs (lncRNAs)-NEAT1/ miR-148a-3p in advancing breast cancer via several cellular functions such as viability, apoptosis and autophagy which still remain unclear
Summary
Breast Cancer (BC) is one of the malignant illness prevalent among women across the globe and is very fatal with an approximated 2.4 million fresh incidences and 523 000 fatalities registered in 2015 [1]. A large body of evidence shows that lncRNA-NEAT1 has been involved is various studies related to breast cancer but the functions of lncRNA-NEAT1/ miR-148a-3p/wnt axis have never been investigated. It has been reported that NEAT1 was involved to have negatively regulated miR-218 expression [13], facilitated cellular development and invasiveness through the miR-211/HMGA2 network [14] and promoted cellular proliferation and EMT in BC progression [15]. In this context the NEAT1 has been explored to enforce oncogenic functions in breast cancer
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