Abstract
ABSTRACTSepsis is a life-threatening condition and often associated with multiple organ failure. Nuclear-enriched abundant transcript 1 (NEAT1), a member of the long non-coding RNAs (lncRNAs), was reported to be involved in the regulation of sepsis progression. However, its precise regulatory mechanism needs to be further explored. In this study, the cell-counting kit-8 assay was used to check cell viability. The quantitative real-time polymerase chain reaction (qRT-PCR) was employed to detect the expression levels of NEAT1, miR-370-3p and Interleukin 1 receptor associated kinase 2 (Irak2). Flow cytometry assay and ELISA were used to check cell apoptosis and the concentrations of inflammatory cytokines, respectively. The starBase was used to predict binding sites between miR-370-3p and NEAT1 or Irak2 and the dual-luciferase reporter assay was performed to verify the interaction. The protein level of Irak2 in samples was measured by western blot. The high concentration of lipopolysaccharide (LPS) led to the high death ratio of RAW 264.7 and HL-1 cells. NEAT1 and Irak2 were upregulated in sepsis tissues and LPS-induced RAW 264.7 and HL-1 cells, opposite to the expression of miR-370-3p. In addition, knockdown of NEAT1 promoted viability, suppressed apoptosis and reduced the expression of inflammatory cytokines in LPS-induced RAW 264.7 and HL-1 cells. Moreover, we found that miR-370-3p interacted with NEAT1 and targeted the 3′UTR of Irak2. Further research indicated that downregulation of miR-370-3p or upregulation of Irak2 rescued NEAT1 silencing-mediated inhibitory effect on sepsis progression. Knockdown of NEAT1 hampered sepsis progression by downregulating Irak2 via interacting with miR-370-3p in LPS-induced RAW 264.7 and HL-1 cells.
Highlights
Sepsis is a life-threatening condition and often associated with multiple organ failure
Previous reports demonstrated that long non-coding RNAs (lncRNAs) nuclear-enriched abundant transcript 1 (NEAT1) was involved in the development of diverse human cancers, including myeloma (Taiana et al, 2019), breast cancer
Though these studies showed that Nuclear-enriched abundant transcript 1 (NEAT1) was closely associated with sepsis-induced injury, the regulatory mechanism of NEAT1 in sepsis progression is still worth studying
Summary
Sepsis is a life-threatening condition and often associated with multiple organ failure. The qRT-PCR was employed to detect the expression levels of NEAT1, miR-370-3p and iIrak. Flow cytometry assay and ELISA were used to check cell apoptosis and the concentrations of inflammatory cytokines, respectively. The starBase was used to predict binding sites between miR-370-3p and NEAT1 or Irak and the dual-luciferase reporter assay was performed to verify the interaction. NEAT1 and Irak were upregulated in sepsis tissues and LPS-induced RAW 264.7 and HL-1 cells, opposite to the expression of miR-370-3p. Knockdown of NEAT1 promoted viability, suppressed apoptosis and reduced the expression of inflammatory cytokines in LPS-induced RAW 264.7 and HL-1 cells. We found that miR-370-3p interacted with NEAT1 and targeted the 3’UTR of Irak. Further research indicated that downregulation of miR-370-3p or upregulation of IraK2 rescued NEAT1 silencing-mediated inhibitory effect on sepsis progression
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