Abstract
BackgroundLncRNA NEAT1 promotes inflammatory responses, which contribute to recurrent aphthous stomatitis (RAS). This study focused on the involvement of NEAT1 in RAS.MethodsRT-qPCR and ELISA were performed to determine the expression of NEAT1 and proinflammatory factors (IL-2, IL-1β, and TNF-α) in plasma from patients with a history of RAS and showing symptom (n = 80, S-RAS group), people with a history of RAS but showing no symptom (n = 80, NS-RAS group), and controls without a history of RAS (n = 80, Control group). Correlation analysis was performed with Pearson’s correlation coefficient. S-RAS group received treatmen,t and plasma levels of NEAT1 and proinflammatory factors were compared before and after treatment. S-RAS group was followed up for 12 months, and the recurrence was recorded.ResultsPlasma NEAT1, IL-2, IL-1β, and TNF-α levels were the highest in the S-RAS group, followed in turn by NS-RAS and control groups. NEAT1 was positively and significantly correlated with IL-2, IL-1β, and TNF-α across S-RAS and NS-RAS samples, but not control samples. After treatment, plasma levels of NEAT1, IL-2, IL-1β, and TNF-α decreased significantly. Moreover, a higher recurrence rate was observed during the follow-up in patients with high plasma NEAT1 levels.ConclusionNEAT1 is upregulated in RAS and correlated with multiple proinflammatory factors. Moreover, NEAT1 has predictive values for RAS.
Highlights
LncRNA NEAT1 promotes inflammatory responses, which contribute to recurrent aphthous stomatitis (RAS)
Analysis of plasma levels of NEAT1, IL‐2, IL‐1β, and TNF‐α in three groups of participants Plasma samples from the S-RAS, NS-RAS and control groups were subjected to reverse transcriptions (RTs)-qPCR and ELISA to determine the plasma levels of NEAT1, IL-2, IL-1β, and TNFα
Our results illustrated that plasma levels of NEAT1 (Fig. 1a, p < 0.01), IL-2 (Fig. 1b, p < 0.01), IL-1β (Fig. 1c, p < 0.01), and TNF-α (Fig. 1d, p < 0.01) were the highest in the S-RAS group, followed in turn by the NS-RAS and control groups
Summary
LncRNA NEAT1 promotes inflammatory responses, which contribute to recurrent aphthous stomatitis (RAS). This study focused on the involvement of NEAT1 in RAS. Recurrent aphthous stomatitis (RAS), presenting as shallow, painful round ulcer with yellowish-gray center and erythematous margin (well-defined), is the most frequently diagnosed oral mucosa ulcerative disease [1, 2]. The incidence of RAS varies a lot across different populations. About 2–66% of the population are suffering from RAS [3]. RAS is closely correlated with brushing habits, brushing time, other oral diseases, age, RAS is an inflammatory condition in oral mucosa with the involvement of multiple inflammatory factors, such as IL-2, IL-1β, and TNF-α [8–10]. Controlling the production of inflammatory responses is critical in the treatment of RAS.
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