Abstract
Background LncRNA MSC-AS1 has been reported to be a tumor promoter in hepatocellular carcinoma. However, the function of MSC-AS1 in colorectal cancer (CRC) has not been elucidated. It is designed to study the expression level of MSC-AS1 and investigate its biological effect on the progression of CRC. Methods The expression patterns of MSC-AS1, miR-325, and TRIM14 were explored by RT-qPCR in CRC tissues and cells. The protein expression of TRIM14 was tested by Western blot assay. The association between MSC-AS1 expression and clinicopathological data was analyzed by chi-squared test. CCK-8 assay, colony formation, and Transwell assay were used to investigate the effect of MSC-AS1 on cell growth, invasion, and migration in CRC cells. The correlations among MSC-AS1, miR-325, and TRIM14 were analyzed by Pearson's correlation coefficient analysis. Results We found that MSC-AS1 and TRIM14 were upregulated in CRC tissues, while miR-325 was downregulated in CRC tissues. Functional experiments demonstrated that MSC-AS1 knockdown inhibited cell proliferation, migration, and invasion abilities in CRC cells. Additionally, miR-325 was proved to be a target miRNA of MSC-AS1, and TRIM14 might be a downstream gene of miR-325. Besides that, MSC-AS1 counteracted the inhibitory effect of miR-325 on the cell progression and TRIM14 expression. Conclusion Our results indicated that MSC-AS1 facilitated CRC progression by sponging miR-325 to upregulate TRIM14 expression. We suggested that MSC-AS1 might be a potential lncRNA-target for CRC therapy.
Highlights
Colorectal cancer (CRC) is one of the most common gastrointestinal malignancies, which seriously threatens human health. e incidence of colorectal cancer (CRC) has been on the rise worldwide
GEPIA database showed that MSC antisense RNA 1 (MSC-AS1) was upregulated in colon adenocarcinoma (COAD) (Figure 1(a))
Studies have found abnormal expression of long noncoding RNAs (lncRNAs) in various human cancers, which play a key role in tumor development, metastasis, early diagnosis, prognosis evaluation, radiotherapy and chemotherapy efficacy, etc
Summary
Colorectal cancer (CRC) is one of the most common gastrointestinal malignancies, which seriously threatens human health. e incidence of CRC has been on the rise worldwide. LncNEAT1 was reported to promote CRC progression by suppressing miR-486-5p expression and regulating NR4A1/Wnt/β-catenin pathway [8]. MSC-AS1 promoted tumor progression in kidney renal clear cell carcinoma [12]. LncRNA MSC-AS1 has been reported to be a tumor promoter in hepatocellular carcinoma. It is designed to study the expression level of MSC-AS1 and investigate its biological effect on the progression of CRC. E expression patterns of MSC-AS1, miR-325, and TRIM14 were explored by RT-qPCR in CRC tissues and cells. We found that MSC-AS1 and TRIM14 were upregulated in CRC tissues, while miR-325 was downregulated in CRC tissues. MSC-AS1 counteracted the inhibitory effect of miR-325 on the cell progression and TRIM14 expression. Our results indicated that MSC-AS1 facilitated CRC progression by sponging miR-325 to upregulate TRIM14 expression. We suggested that MSC-AS1 might be a potential lncRNA-target for CRC therapy
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