Abstract
PurposeOvarian cancer (OC) is the most common malignancy in women with high mortality. Increasing studies have revealed that long non-coding RNA (lncRNA) MNX1-AS1 has a promoting effect on various cancers. However, the mechanisms of MNX1-AS1 in OC are still unclear. Therefore, this study focused on exploring the mechanisms of MNX1-AS1 in OC.Materials and methodsThe expression of SOX12 at the protein level was detected by western blot. Cell proliferation was detected by CCK8 assay and colony formation assay. Cell cycle and cell apoptosis were detected by flow cytometry. Wound-healing assay, transwell assay and western blot were used to detect the ability of cell migration and invasion. The target binding was confirmed through the luciferase reporter assay.ResultsThe expression of MNX1-AS1 was increased in OC tumor tissues and cells. Elevated MNX1-AS1 expression is associated with advanced stage and lower overall survival rate. Knockdown of MNX1-AS1 inhibited cell proliferation, migration and invasion, blocked cell cycle, and promoted cell apoptosis in SKOV-3 and OVCAR-3 cells. MNX1-AS1 was competitively binding with miR-744-5p, and its downstream target gene was SOX12. miR-544-5p expression was decreased, while SOX12 expression was increased in OC tumor tissues and cells. Overexpression of miR-744-5p inhibited cell proliferation, migration, invasion and promoted cell apoptosis in SKOV-3 and OVCAR-3 cells.ConclusionMNX1-AS1 promoted the development of OC through miR-744-5p/SOX12 axis. This study revealed a novel mechanism of MNX1-AS1 in OC, which may provide a new treatment or scanning target for OC.
Highlights
Ovarian cancer (OC) is the most common malignancy in women [1]
motor neuron and pancreas homeobox protein 1 (MNX1)-AS1 was competitively binding with miR-744-5p, and its downstream target gene was SOX12. miR-544-5p expression was decreased, while SOX12 expression was increased in OC tumor tissues and cells
MNX1-AS1 promoted the development of OC through miR-744-5p/SOX12 axis
Summary
Ovarian cancer (OC) is the most common malignancy in women [1]. More than 70% of OC patients are diagnosed at advanced stages (III and IV stage) due to early non-specific symptoms and a lack of effective screening methods [2]. It is the main reason the 5-year survival rate of patients with OC is still only 20–40%, the treatment methods such as surgery, radiotherapy. Shen et al Journal of Ovarian Research (2021) 14:161 cancer, and it promotes cell proliferation and migration through sponging of miR-215 and further regulating the expression of CDC6 [8]. LncRNA MT1JP expression is decreased in gastric cancer, and it inhibits the process of gastric cancer through competitively binding to miR-92a-3p and regulating the expression of FBXW7 [9]
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