Abstract
BackgroundHepatoblastoma (HB) is identified to be the most common liver malignancy which occurs in children. Long non-coding RNAs (lncRNAs) have been implicated in numerous biological processes and diseases, including HB. LncRNA MIR205 host gene (MIR205HG) has been investigated in multiple cancers, however, its role in HB remains to be elucidated.MethodsMIR205HG expression was analyzed by RT-qPCR. EdU, colony formation and transwell assays were implemented to measure the biological function of MIR205HG on the progression of HB. Mechanism assays were carried out to probe into the underlying mechanism of MIR205HG in HB cells.ResultsMIR205HG was significantly overexpressed in HB. Moreover, MIR205HG inhibition suppressed the proliferative, migratory and invasive capacities of HB cells. Furthermore, MIR205HG competitively bound to microRNA-514a-5p (miR-514a-5p) and targeted mitogen-activated protein kinase 9 (MAPK9) to stimulate mitogen activated protein kinase (MAPK) signaling pathway. Besides, MIR205HG also served as a sponge for microRNA-205-5p (miR-205-5p) to activate the PI3K/AKT signaling pathway.ConclusionMIR205HG drives the progression of HB which might provide an efficient marker and new therapeutic target for HB.
Highlights
Hepatoblastoma (HB) is identified to be the most common liver malignancy which occurs in children
LncRNA MIR205HG is overexpressed in HB and mainly located in cytoplasm of HB cells According to GEO database, we discovered that Long non-coding RNAs (lncRNAs) MIR205HG was up-regulated in HB tissues
Quantitative real-time PCR (RT-qPCR) was used to examine MIR205HG expression in HepG2, HuH-7, HuH-1, HuH-6 and THLE-3 cell lines, and the results suggested that MIR205HG expression was distinctly high in HB cell lines (HepG2, HuH-7, HuH-1, HuH-6) compared to normal cell line (THLE-3) (Fig. 1A)
Summary
Hepatoblastoma (HB) is identified to be the most common liver malignancy which occurs in children. Long non-coding RNAs (lncRNAs) have been implicated in numerous biological processes and diseases, including HB. LncRNA MIR205 host gene (MIR205HG) has been investigated in multiple cancers, its role in HB remains to be elucidated. HB is the most common primary liver tumor which frequently occurs in infants and in children younger than 5 years [1]. It accounts for more than 25% pediatric hepatic tumors and almost half of those are malignant [2]. For the treatment of HB, complete surgical removal is of great significance in curing HB. Thence, exploring therapeutic strategies of HB from a novel insight may be helpful for the treatment of HB
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