Abstract
BackgroundNon-small cell lung cancer (NSCLC) is the most common tumor with severe morbidity and high mortality. Long non-coding RNAs (lncRNAs) as crucial regulators participate in multiple cancer progressions. However, the role of lncRNA MEG8 in the development of NSCLC remains unclear. Here, we aimed to investigate the effect of lncRNA MEG8 on the progression of NSCLC and the underlying mechanism.MethodsCell proliferation was analyzed by EdU assays. The impacts of lncRNA MEG8, miR-15a-5p, and miR-15b-5p on cell invasion and migration of NSCLC were assessed by transwell assay. The luciferase reporter gene assay was performed using the Dual-luciferase Reporter Assay System. The effect of lncRNA MEG8, miR-15a-5p, and miR-15b-5p on tumor growth was evaluated in nude mice of Balb/c in vivo.ResultsWe revealed that the expression levels of MEG8 were elevated in the NSCLC patient tissues compared to that in adjacent normal tissues. The expression of MEG8 was negatively relative to that of miR-15a-5p and miR-15b-5p in the NSCLC patient tissues. The expression of MEG8 was upregulated, while miR-15a-5p and miR-15b-5p were downregulated in NSCLC cell lines. The depletion of MEG8 inhibited NSCLC cell proliferation, migration, and invasion in vitro. MEG8 contributed to NSCLC progression by targeting miR-15a-5p/miR-15b-5p in vitro. LncRNA MEG8 contributes to tumor growth of NSCLC via the miR-15a/b-5p/PSAT1 axis in vivo. Thus, we concluded that lncRNA MEG8 promotes NSCLC progression by modulating the miR-15a/b-5p/PSAT1 axis.ConclusionsOur findings demonstrated that lncRNA MEG8 plays a critical role in NSCLC development. LncRNA MEG8, miR-15a-5p, miR-15b-5p, and PSAT1 may serve as potential targets for NSCLC therapy.
Highlights
Non-small cell lung cancer (NSCLC) is the most common tumor with severe morbidity and high mortality
MEG8 is up‐regulated and miR‐15a‐5p/miR‐15b‐5p is down‐regulated in NSCLC patients and NSCLC cell lines To assess the potential correlation of Long non-coding RNAs (lncRNAs) MEG8 and miR-15a/b-5p with NSCLC progression, their expression was evaluated in NSCLC patients and NSCLC cell lines
MEG8 contributes to tumor growth of NSCLC via miR‐15a/ b‐5p/Phosphoserine aminotransferase 1 (PSAT1) axis in vivo We further evaluated the effect of lncRNA MEG8-miR15a-5p/miR-15b-5p axis on NSCLC development in vivo
Summary
Non-small cell lung cancer (NSCLC) is the most common tumor with severe morbidity and high mortality. Long non-coding RNAs (lncRNAs) as crucial regulators participate in multiple cancer progressions. We aimed to investigate the effect of lncRNA MEG8 on the progression of NSCLC and the underlying mechanism. Long non-coding RNAs (lncRNAs) as the emerging critical biological regulators, play critical roles in many physiological and pathological processes such as cell cycle, differentiation, apoptosis, cardiovascular diseases, and cancer progression [5, 6]. LncRNA MEG8 is a poor-investigated lncRNAs. It has been reported that lncRNA MEG8 enhances the epithelial-mesenchymal transition by modulating epigenetic progression in pancreatic and lung cancer cells [10, 11]. LncRNA MEG8 is abnormally expressed in lung cancer tissues [12]. The effect of lncRNA MEG8 on NSCLC progression and the underlying mechanism are unclear
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