Abstract
Dietary restriction (DR) extends lifespan in many species which is a well-known phenomenon. Long non-coding RNAs (lncRNAs) play an important role in regulation of cell senescence and important age-related signaling pathways. Here, we profiled the lncRNA and mRNA transcriptome of fruit flies at 7 day and 42 day during DR and fully-fed conditions, respectively. In general, 102 differentially expressed lncRNAs and 1406 differentially expressed coding genes were identified. Most informatively we found a large number of differentially expressed lncRNAs and their targets enriched in GO and KEGG analysis. We discovered some new aging related signaling pathways during DR, such as hippo signaling pathway-fly, phototransduction-fly and protein processing in endoplasmic reticulum etc. Novel lncRNAs XLOC_092363 and XLOC_166557 are found to be located in 10 kb upstream sequences of hairy and ems promoters, respectively. Furthermore, tissue specificity of some novel lncRNAs had been analyzed at 7 day of DR in fly head, gut and fat body. Also the silencing of lncRNA XLOC_076307 resulted in altered expression level of its targets including Gadd45 (involved in FoxO signaling pathway). Together, the results implicated many lncRNAs closely associated with dietary restriction, which could provide a resource for lncRNA in aging and age-related disease field.
Highlights
Dietary restriction (DR) is the best and nongenetic antiaging strategy without malnutrition, aiming to intervene on environmental factors and reduce the risk of many age-related diseases [1]
A substantial number of DR-specific Long non‐coding RNAs (lncRNAs) and corresponding targets involved in multiple signaling pathways were identified in this study
We have predicted potential functions for novel lncRNA based on target function enrichment and found important known lncRNA and their targets having the tissue specificity
Summary
Dietary restriction (DR) is the best and nongenetic antiaging strategy without malnutrition, aiming to intervene on environmental factors and reduce the risk of many age-related diseases [1]. DR has been shown to extend lifespan and improve health with the most consistent non-pharmacological intervention during aging in diverse organisms, such as flies [2], yeast [3], nematodes [4] and rodents [5]. These universal effects suggest that there should be some conserved common potential genetic pathways and biochemical processes by virtue of which different organisms delay aging in response to DR [2]. While lncRNAs have significant functions and effect on the course of aging, the number of lncRNAs and their functional implications in anti-aging process are not yet well validated during nutritional interventions especially during DR
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