Abstract
Infantile hemangioma (IH) is a common benign tumor of endothelial cells in infants. Most hemangiomas are self-limited, but a few may develop and lead to serious complications that affect the normal life of children. Therefore, finding an effective treatment strategy for IH is a pressing need. Recent studies have demonstrated that non-coding RNAs affect the progression of multiple tumors. This study aims to investigate the mechanism by which LncRNA-MCM3AP-AS1 promotes glycolysis in the pathogenesis of IH. We first documented that the expression of LncRNA MCM3AP-AS1 was significantly upregulated in IH. Furthermore, we demonstrated that MCM3AP-AS1 bound to miR-106b-3p which promotes glycolysis in IH. In addition, we found that inhibition of HIF-1α contributed to the transformation of glycolysis to normal aerobic oxidation, partially reversed the promoting effect on glycolysis by the up-regulation of LncRNA MCM3AP-AS1 in IH disease. More importantly, we demonstrated this phenomenon existed in IH patients. Taken together, we demonstrate that LncRNA-MCM3AP-AS1 promotes the progression of infantile hemangiomas by increasing the glycolysis via regulating miR-138-5p/HIF-1α axis.
Highlights
Infantile hemangiomas (IH) is a common benign skin tumor in children with vascular endothelial cell proliferation as the main pathological characteristic (Chamli et al, 2021)
The results showed that among a variety of Long non-coding RNAs (lncRNAs), only LncRNA MCM3AP-AS1 was up-regulated in IH compared to normal tissue (Figure 1A)
We compared the expression of these lncRNAs in hemangioma cell lines and normal cell lines, and our experimental results demonstrated that the expression of LncRNA MCM3AP-AS1 was significantly up-regulated in HemECs compared to the HUVECs that was taken as controls (Figure 1B)
Summary
Infantile hemangiomas (IH) is a common benign skin tumor in children with vascular endothelial cell proliferation as the main pathological characteristic (Chamli et al, 2021). Compared with localized IH lesions, deep lesions are at greater risk of ulceration and dysfunction, and require early and aggressive clinical intervention and treatment (Cheng and Friedlander, 2016). Most of the subsided tumors will leave scar or fibrous fat deposition, early active intervention and treatment would be preferred. The tendency of spontaneous regression is an important feature of the course of IH, and its pathological basis is the disappearance of juvenile capillary degeneration, replaced by deposition of fiber and adipose tissues (Caussé et al, 2013). Promoting the transition from early stage to regression stage is the main goal of current treatment
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