Abstract
BackgroundIt is known that Fas ligand (FasL) is involved in the development of intervertebral disc degeneration (IDD). A recent study reported that lncRNA MAGI2-AS3 up-regulated the expression of FasL to promote breast cancer. Therefore, we investigated the roles that lncRNA MAGI2-AS3 might play in IDD.MethodsA total of 66 IDD patients (IDD group) and 58 healthy volunteers (Control group) were recruited in this study. Quantitative real-time PCR (qRT-PCR) and western blot were used to investigate gene expression levels. Cell transfections were carried out to analyze gene interactions. The diagnostic value of lncRNA MAGI2-AS3 for IDD was assessed by ROC curve analysis.ResultsThe expression levels of plasma lncRNA MAGI2-AS3 were lower in IDD patients compared to that in the control group. Down-regulation of lncRNA MAGI2-AS3 effectively distinguished IDD patients from the control group. The expression levels of plasma lncRNA MAGI2-AS3 were significantly increased after the treatments. Over-expression of lncRNA MAGI2-AS3 inhibited the expression of FasL, while the silencing of lncRNA MAGI2-AS3 promoted the expression of FasL in nucleus pulposus (NP) cells.ConclusionsTherefore, lncRNA MAGI2-AS3 is down-regulated in IDD and participates in the regulation of FasL expression in nucleus pulposus (NP) cells.
Highlights
It is known that Fas ligand (FasL) is involved in the development of intervertebral disc degeneration (IDD)
Plasma levels of long non-coding RNAs (lncRNAs) MAGI2-AS3 were altered in IDD patients Plasma levels of lncRNA MAGI2-AS were assessed by Quantitative real-time PCR (qRT-PCR) in both IDD patients and healthy people
Plasma levels of lncRNA MAGI2-AS3 were significantly lower in IDD patients (Fig. 1, p < 0.01)
Summary
It is known that Fas ligand (FasL) is involved in the development of intervertebral disc degeneration (IDD). A recent study reported that lncRNA MAGI2-AS3 up-regulated the expression of FasL to promote breast cancer. We investigated the roles that lncRNA MAGI2-AS3 might play in IDD. Intervertebral disc degeneration (IDD) is a common bone disease that causes lower back pain [1]. FasL-induced apoptosis plays essential roles in the development of IDD [8]. LncRNAs are a group of non-protein coding RNAs with critical functions involved in various human diseases [11]. It has been reported that lncRNA MAGI2AS3 can up-regulate FasL to participate in the development of breast cancer [12], indicating the potential involvement of lncRNA MAGI2-AS3 in IDD.
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