Abstract
Background Cervical cancer is the leading cause of cancer deaths among women, and more than 85% of cervical cancer deaths occur in low and middle-income countries. The purpose of this study is to investigate the functions of MAGI2-AS3 and miR-15b in cervical cancer. Materials and Methods The mRNA levels of MAGI2-AS3, miR-15b, and CCNE1 were evaluated using RT-qPCR assay. Dual-luciferase reporter gene assay was used to confirm whether miR-15b binds to CCNE1. Results LncRNA MAGI2-AS3 was downregulated, while miR-15b was upregulated in cervical cancer. Cervical cancer patients with low expression of MAGI2-AS3 have a poor prognosis. Upregulation of MAGI2-AS3 inhibited proliferative and invasive abilities of HeLa cells via regulating the expression of miRNA-15b. MiR-15b inhibitor suppressed cell proliferation and invasion. CCNE1 was a direct target gene of miR-15b, which binds to the 3'-UTR of its mRNA. MiR-15b partially reversed the inhibitory effect of overexpression of MAGI2-AS3 on the proliferation and invasion of HeLa cells. MAGI2-AS3 mediated the expression of CCNE1 in HeLa cells. Conclusion LncRNA MAGI2-AS3 inhibits the proliferation and invasion of cervical cancer cells via the miRNA-15/CCNE1 axis. Our results illustrates that MAGI2-AS3 can be used as a useful clinical predictor for early diagnosis and prognosis assessment of cervical cancer.
Highlights
Cervical cancer (CC) is the fourth most commonly diagnosed cancer in women and one of the most common cancers in the world. ere were approximately 570,000 new cases worldwide in 2018, and 311,000 died of cervical cancer [1, 2]. ere are two main types of cervical cancer: cervical squamous cell carcinoma (CSCC), which accounts for approximately 80% to 85% of cervical cancer cases, and the remainder are cervical adenocarcinoma (CAC) [3]
Treatments such as chemotherapy, radiation, and surgical resection have improved the prognosis of earlystage cervical cancer, the metastasis and recurrence of cervical cancer are the cause of most deaths [4]. us, it is urgent to investigate the biomarkers related to cervical cancer metastasis
Kaplan–Meier curve was employed to evaluate the overall survival of cervical cancer patients, indicating that downregulation of MAGI2-AS3 predicted a worse prognosis (Figure 1(d))
Summary
Cervical cancer (CC) is the fourth most commonly diagnosed cancer in women and one of the most common cancers in the world. ere were approximately 570,000 new cases worldwide in 2018, and 311,000 died of cervical cancer [1, 2]. ere are two main types of cervical cancer: cervical squamous cell carcinoma (CSCC), which accounts for approximately 80% to 85% of cervical cancer cases, and the remainder are cervical adenocarcinoma (CAC) [3]. Ere are two main types of cervical cancer: cervical squamous cell carcinoma (CSCC), which accounts for approximately 80% to 85% of cervical cancer cases, and the remainder are cervical adenocarcinoma (CAC) [3]. Treatments such as chemotherapy, radiation, and surgical resection have improved the prognosis of earlystage cervical cancer, the metastasis and recurrence of cervical cancer are the cause of most deaths [4]. Long noncoding RNA DSCAM-AS1 contributed to tumorigenesis to enhance cell proliferation, migration, and invasion ability in cervical cancer [8]. LncRNA MAGI2-AS3 inhibits the proliferation and invasion of cervical cancer cells via the miRNA-15/CCNE1 axis. Our results illustrates that MAGI2-AS3 can be used as a useful clinical predictor for early diagnosis and prognosis assessment of cervical cancer
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