Abstract
Objective Oral squamous cell carcinoma (OSCC) is the most common malignant tumor of the head and neck, with strong local invasiveness and cervical lymph node metastasis. The purpose of this study was to investigate the role of LINC01296 in oral squamous cell carcinoma and its possible mechanism. Materials and Methods GEPAI database analysis and clinical samples were used to detect the expression of LINC01296 in head and neck cancer. In vivo experiment, MTT, clone formation assay, and transwell were used to detect the proliferation, migration, and invasion of oral squamous cell carcinoma. The effect of LINC01296 on EMT was detected by western blot and qRT-PCR to measure the expression of epithelial and mesenchymal phenotypic markers. BALB/c nude mice were used to carry out in vitro treatment experiment. In terms of mechanism, the binding relationship between LINC01296 and SRSF1 was predicted and verified by the RBPDB database and RNA pull-down assay. Results LINC01296 was highly expressed in clinical samples and cell lines of oral squamous cell carcinoma. Overexpression of LINC01296 promoted the proliferation, invasion, and migration of oral squamous cell carcinoma cells and accelerated the formation of xenografts, while silencing LINC01296 inhibited tumor progression. In mechanism, LINC01296 plays a tumor-promoting role by binding to SRSF1 protein. Conclusion LINC01296 promotes malignant lesions in oral squamous cell carcinoma by binding to SRSF1 protein, which provides important experimental data and theoretical basis for the prevention, diagnosis, and treatment of oral squamous cell carcinoma.
Highlights
Head and neck cancer (HNC) is the sixth most common malignant tumor in the world
We examined the carcinomatous tissues and paracancerous tissues of 58 patients with Oral squamous cell carcinoma (OSCC) by PCR and found that LINC01296 was significantly increased in OSCC tissues (Figure 1(d))
By comparing normal cells and 6 kinds of oral squamous cell carcinoma lines, the results revealed that LINC01296 was highly expressed in OSCC cell lines (Figure 1(e))
Summary
Head and neck cancer (HNC) is the sixth most common malignant tumor in the world. Oral squamous cell carcinoma (OSCC) is the most common subtype of head and neck cancer [1]. The main methods for the treatment of OSCC are surgical resection, adjuvant radiotherapy, and chemotherapy. Great progress has been made in surgical techniques and radiotherapy and chemotherapy, in the past 30 years, the overall survival rate of patients with OSCC has been maintained at about 50% and has not been significantly improved [3]. In-depth study of the molecular mechanism of the occurrence and development of OSCC is the key to the development of new and effective treatments
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