Abstract
BackgroundLncRNA was known to be closely associated with the progression of human tumors. The role of lncRNA LIFR-AS1 in the pathogenesis and progression of gastric tumor is still unclear. The aim of this study was to investigate the function of LIFR-AS1 and the underlying mechanism in the pathogenesis and progression of gastric cancer.MethodsQRT-PCR was used to evaluate the expression of LIFR-AS1, miR-29a-3p and COL1A2 in gastric tumor tissues and cells. Western blotting was used to evaluate the protein expression of COL1A2 in gastric tumor cells. CCK-8 assay, transwell assay and flow cytometry were used to evaluate the roles of LIFR-AS1, miR-29a-3p and COL1A2 in cell proliferation, invasion, migration and apoptosis. The relationship among LIFR-AS1, miR-29a-3p and COL1A2 was assessed by bioinformatics analyses and luciferase reporter assay.ResultsThe expression levels of LIFR-AS1 were significantly increased in gastric tumor tissues and cells, while the expression levels of miR-29a-3p were decreased. The expression of miR-29a-3p was negatively correlated with the expression of LIFR-AS1 in gastric cancer tumor tissues. Knocking down of LIFR-AS1 inhibited proliferation, invasion and migration of gastric tumor cells, and induced apoptosis of gastric tumor cells. Bioinformatics analyses and integrated experiments revealed that LIFR-AS1 elevated the expression of COL1A2 through sponging miR-29a-3p, which further resulted in the progression of gastric tumor.ConclusionLIFR-AS1 plays an important role as a competing endogenous RNA in gastric tumor pathogenesis and may be a potential target for the diagnosis and treatment of gastric tumor.
Highlights
LncRNA was known to be closely associated with the progression of human tumors
After down-regulating the expression of LIFR-AS1 in BSG823 cells and BGC803 cells with shLIFR-AS1(Fig. 1c, p < 0.05), the cell viability, colony formation, invasion and migration of BSG823 cells and BGC803 cells were all significantly decreased (Fig. 1d–f, p < 0.05), while the apoptosis of BSG823 cells and BGC803 cells was significantly increased (Fig. 1g, p < 0.01). These results suggested that LIFR-AS1 played an important role in the progression of gastric cancer (GC)
After up-regulating the expression of miR-29a-3p in BSG823 cells and BGC803 cells with miR-29a-3p mimic (Fig. 2d, p < 0.05), the cell viability, colony formation, invasion and migration of BSG823 cells and BGC803 cells were all significantly decreased (Fig. 2e–g, p < 0.05), while the apoptosis of BSG823 cells and BGC803 cells was significantly increased (Fig. 2h, p < 0.01). These results suggested that miR-29a-3p played an important role in the progression of GC
Summary
The role of lncRNA LIFR-AS1 in the pathogenesis and progression of gastric tumor is still unclear. The aim of this study was to investigate the function of LIFR-AS1 and the underlying mechanism in the pathogenesis and progression of gastric cancer. The incidence and mortality of GC is the fifth and the third, respectively [3] It is seriously affecting the quality of life. Pan et al Cancer Cell Int (2021) 21:7 of lncRNAs in the pathogenesis and progression of GC is still unclear. Another study reported that ALKBH5 promotes invasion and metastasis of gastric cancer by decreasing methylation of lncRNA NEAT1, suggesting that lncRNAs might serve as potential therapeutic targets for GC [11]. The function of LIFR -AS1 in GC has not been studied
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