Abstract
BackgroundNon-small cell lung cancer (NSCLC) is the most deadly cancer worldwide. LncRNA KCNQ1OT1 has been reported to be involved in the progression of various tumors, including NSCLC. However, the precise mechanism of KCNQ1OT1 in NSCLC requires further investigation.MethodsThe expression levels of KCNQ1OT1, miR-129-5p and JAG1 were detected by qRT-PCR or western blot. Kaplan–Meier survival analysis was used to assess the correlation between KCNQ1OT1 expression and the overall survival of NSCLC patients. CCK-8 assay was used to measure cell viability. Cell migration and invasion were detected by transwell assay. The targets of KCNQ1OT1 and miR-129-5p were predicted by bioinformatics, which was confirmed by dual-luciferase reporter assay or pull-down assay.ResultsKCNQ1OT1 expression was significantly enhanced, while miR-129-5p expression was dramatically reduced in NSCLC tissues and cells. Higher KCNQ1OT1 shortened overall survival and was positively associated with tumor stage and lymph node metastasis. KCNQ1OT1 knockdown inhibited proliferation, migration and invasion of NSCLC cells. Inhibition of miR-129-5p attenuated the inhibition of NSCLC cell viability, migration and invasion induced by KCNQ1OT1 knockdown. In addition, JAG1 was confirmed as a target of miR-129-5p. Knockdown of JAG1 reversed the effects of miR-129-5p knockdown on NSCLC progression. KCNQ1OT1 regulated JAG1 expression by sponging miR-129-5p in NSCLC cells.ConclusionKCNQ1OT1 induced proliferation, migration and invasion of NSCLC cells by sponging miR-129-5p and regulating JAG1 expression, indicating that KCNQ1OT1 was a therapeutic target for NSCLC.
Highlights
Non-small cell lung cancer (NSCLC) is the most deadly cancer worldwide
KCNQ1OT1 was upregulated in NSCLC tissues and cells and correlated with poor prognosis In order to verify the differential expression of KCNQ1OT1 in NSCLC tissues and cells, qRT-PCR was performed to detect expression levels
The results revealed that KCNQ1OT1 expression was significantly upregulated in NSCLC tissues compared to adjacent non-tumor tissues (Fig. 1a)
Summary
Non-small cell lung cancer (NSCLC) is the most deadly cancer worldwide. LncRNA KCNQ1OT1 has been reported to be involved in the progression of various tumors, including NSCLC. Lung cancer is the most deadly cancer in the world with high morbidity and mortality [1]. Lung cancer is divided into two subtypes of non-small cell lung cancer (NSCLC). Long noncoding RNAs (lncRNAs) are non-protein coding transcripts longer than 200 nucleotides in length [4]. Increasing evidence has shown that lncRNAs exerted significant regulatory effects on various biological processes and gene expression through multiple mechanisms [5]. KCNQ1 overlapping transcript 1 (KCNQ1OT1) was involved in the regulation of numerous genes within the kcnq domain [6]. KCNQ1OT1 facilitated progression of cholangiocarcinoma (CCA) by sponging miR-140-5p and regulating SOX4 expression [10]. The mechanism of KCNQ1OT1 in NSCLC still needs further research
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