LncRNA IMFlnc1 promotes porcine intramuscular adipocyte adipogenesis by sponging miR-199a-5p to up-regulate CAV-1

Jing Wang,Jia-Qing Zhang,Bao-Song Xing,Hai Cao,Chuan-Ying Pan,Ming-Yue Chen,Jun-Feng Chen,Qiao-Ling Ren

doi:10.1186/s12860-020-00324-8

Abstract

BackgroundLocal Chinese local pig breeds have thinner muscle fiber and higher intramuscular-fat (IMF) content. But its regulation mechanism has not been discussed in-depth. Studies indicated that long non coding RNAs (lncRNAs) play important role in muscle and fat development.ResultsThe lncRNAs expressional differences in the longissimus dorsi (LD) muscle were identified between Huainan pigs (local Chinese pigs, fat-type, HN) and Large White pigs (lean-type, LW) at 38, 58, and 78 days post conception (dpc). In total, 2131 novel lncRNAs were identified in 18 samples, and 291, 305, and 683 differentially expressed lncRNAs (DELs) were found between these two breeds at three stages, respectively. The mRNAs that co-expressed with these DELs were used for GO and KEGG analysis, and the results showed that muscle development and energy metabolism were more active at 58 dpc in HN, but at 78 dpc in LW pigs. Muscle cell differentiation and myofibril assembly might associated with earlier myogenesis and primary-muscle-fiber assembly in HN, and cell proliferation, insulin, and the MAPK pathway might be contribute to longer proliferation and elevated energy metabolism in LW pigs at 78 dpc. The PI3K/Akt and cAMP pathways were associated with higher IMF deposition in HN. Intramuscular fat deposition-associated long noncoding RNA 1 (IMFlnc1) was selected for functional verification, and results indicated that it regulated the expressional level of caveolin-1 (CAV-1) by acting as competing endogenous RNA (ceRNA) to sponge miR-199a-5p.ConclusionsOur data contributed to understanding the role of lncRNAs in porcine-muscle development and IMF deposition, and provided valuable information for improving pig-meat quality.

Highlights

Local Chinese local pig breeds have thinner muscle fiber and higher intramuscular-fat (IMF) content
Results long non coding RNAs (lncRNAs) identification in porcine longissimus dorsi (LD) muscle To verify the effect of lncRNAs in LD muscle development during porcine embryonic period, we examined the differentially expressed lncRNAs (DELs) of LD muscles between HN and Large White (LW) pigs at the three embryonic-development stages
LncRNA properties in porcine LD muscle In total, 2131 novel lncRNAs and 2057 annotated lncRNAs were detected from 18 LD muscle samples (Fig. 1a), and only lincRNA (89.9%)and intronic lncRNA were found in the novel lncRNAs (Fig. 1b)

Summary

Introduction

Local Chinese local pig breeds have thinner muscle fiber and higher intramuscular-fat (IMF) content. The diameter of muscle fibers, the content of intramuscular fat, and muscle fiber types are all involved in regulating meat-quality traits [1, 2]. Meat-quality traits are regulated by nutrition, feeding conditions, stress, and, most importantly, by genetic influence [3]. Lean-type pigs like Large White (LW), Landrace (LR), and Duroc generally have advantages such as high meat production, a fast growth rate, and a low feed-tomeat ratio. Most local Chinese pig breeds are fat-type, and they generally have bright red meat, thin muscle fibers, high intramuscular fat content, and more glycolytic muscle fibers. Two pig types with differences in meatquality traits were a potentially good model to study the genetic-regulation mechanism of potential musclephenotype differences [4]

Methods

Results

Discussion

Conclusion