Abstract
Accumulating studies supported that lncRNAs played important roles in tumorigenesis. LncRNA HOXA11‐AS was a novel lncRNA that has been proved to involved in several tumours. However, the role of HOXA11‐AS in the development of hepatocellular carcinoma (HCC) remains to be explained. In our study, we showed that HOXA11‐AS expression was up‐regulated in the HCC tissues, and the higher expression of HOXA11‐AS was associated with the advanced stage in the HCC samples. In addition, we indicated that the expression of HOXA11‐AS was up‐regulated in HCC cell lines (Hep3B, SMMC‐7721, MHCC97‐H and BEL‐7402) compared with normal liver cell lines (HL‐7702). Overexpression of HOXA11‐AS promoted HCC proliferation and invasion and induced the epithelial‐mesenchymal transition (EMT) and knockdown of HOXA11‐AS suppressed the HCC cell proliferation and invasion. However, we showed that miR‐214‐3p expression was down‐regulated in the HCC tissues and cell lines. Ectopic expression of miR‐214‐3p suppressed HCC cell proliferation and invasion. Furthermore, we indicated that overexpression of HOXA11‐AS decreased the miR‐214‐3p expression and the expression of miR‐214‐3p was negatively related with the HOXA11‐AS expression in HCC samples. Ectopic expression of HOXA11‐AS increased HCC proliferation and invasion and induced EMT through inhibiting miR‐214‐3p expression. These data suggested that HOXA11‐AS/miR‐214‐3p axis was responsible for development of HCC.
Highlights
Hepatocellular carcinoma (HCC) is one of most common tumour worldwide and is the 2nd leading cause of cancer-related death.[1-5]
We demonstrated that the expression of HOXA11-AS was up-regulated in the HCC samples and the higher expression of HOXA11-AS was associated with the advanced stage in the HCC samples
We showed that miR-214-3p expression was downregulated in the HCC tissues and the lower expression of miR-214-3p was correlated with advanced stage in the HCC tissues
Summary
Hepatocellular carcinoma (HCC) is one of most common tumour worldwide and is the 2nd leading cause of cancer-related death.[1-5]. Hepatocarcinogenesis was one complicated procedure and that several factors were involved in the initiation, development and progression of this disease.[12-14]. The detail molecular mechanism of HCC remains largely unknown.[15]. LncRNAs can serve as oncogenes or tumour suppressor genes via crosstalk with other RNA or by several chromatin-based mechanisms.[21-23]. The expression of lncRNAs was found to be deregulated in a large number of tumours including lung cancer, osteosarcoma, ovarian cancer, breast cancer, gastric cancer, colorectal cancer and HCC.[24-30]. LncRNA HOXA11-AS was a novel lncRNA that has been proved to involved in development of some tumours such as gastric cancer, lung cancer and osteosarcoma.[31-33]. The role of lncRNA HOXA11-AS in the HCC remains largely unknown. We investigated the expression and the functional role of HOXA11-AS in HCC. We demonstrated that the expression of HOXA11-AS was down-regulated in HCC cell lines and tissues and overexpression of HOXA11-AS promoted HCC growth, invasion and epithelial-mesenchymal transition (EMT)
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