Abstract
BackgroundLncRNA HOXA-AS2 has been found in the literature to deteriorate glioblastoma. However, its regulatory mechanism is yet to be fully investigated. Our study focused chiefly on the interaction and role of the HOXA-AS2/miR-885-5p/RBBP4 axis in the development of glioblastoma.MethodsqRT-PCR analysis was performed to detect the expression of lncRNA, miRNA and mRNA in glioblastoma tissues and cells. Dual-luciferase assay, RIP assay and RNA pull-down assay were later carried out to reveal the interactions among HOXA-AS2, miR-885-5p and RBBP4. After that, CCK-8 assay, BrdU assay, nude mice xenografting assay, western blot assay, and flow cytometry were carried out to analyze the effect of the HOXA-AS2/miR-885-5p/RBBP4 axis on glioblastoma samples.ResultsHOXA-AS2 and RBBP4 were found to be overexpressed in glioblastoma. Experimental results showed that HOXA-AS2 and RBBP4 contributed to the tumorigenesis of glioblastoma cells. However, miR-885-5p was observed to be downregulated in glioblastoma. Findings also indicated that HOXA-AS2 could negatively regulate miR-885-5p, thereby enhancing RBBP4 expression.ConclusionOverall, HOXA-AS2 promoted the tumorigenesis of glioblastoma by targeting and regulating miR-885-5p to induce the expression of RBBP4.
Highlights
LncRNA HOXA-AS2 has been found in the literature to deteriorate glioblastoma
HOXA-AS2 was found to be overexpressed in glioblastoma tissues and cells, to inhibit cell apoptosis, and to enhance cell proliferation, migration, invasion and the protein expression of Twist, Slug, Vimentin and MMP-2
Our study showed that HOXA-AS2 upregulation occurred in glioblastoma tissues, thereby increasing the aggressiveness of glioblastoma cells
Summary
LncRNA HOXA-AS2 has been found in the literature to deteriorate glioblastoma. Chemotherapy and other surgical techniques have been used to treat glioblastoma patients [4,5,6,7], they do not guarantee the long-term survival of victims. By investigating the underlying pathogenesis of glioblastoma, it will be feasible to discover new diagnoses and treatment methods that can improve the chances of survival of glioblastoma patients in the long-term. Long non-coding RNAs (lncRNAs) can be referred to as non-coding RNA molecules with more than 200 nucleotides. HOXA cluster antisense RNA 2 (HOXAAS2), a long non-coding RNA, is directly involved in such cellular processes as cell proliferation and gene expression. HOXA-AS2 has even been found to play a carcinogenic role in the
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
