LncRNA HCG18 promotes osteosarcoma growth by enhanced aerobic glycolysis via the miR-365a-3p/PGK1 axis

Xiaohui Pan,Zhanpeng Pan,Canjun Liu,Jishan Yuan,Xiang Yao,Zhicheng Yang,Jin Guo

doi:10.1186/s11658-021-00304-6

Abstract

BackgroundOsteosarcoma (OS) is a common primary bone malignancy. Long noncoding RNA HCG18 is known to play an important role in a variety of cancers. However, its role in OS and relevant molecular mechanisms are unclear.MethodsReal-time quantitative PCR was performed to determine the expression of target genes. Function experiments showed the effects of HCG18 and miR-365a-3p on OS cell growth.ResultsHCG18 expression was increased in OS cell lines. Moreover, in vitro and in vivo experiments demonstrated that HCG18 knockdown inhibited OS cell proliferation. Mechanistically, HCG18 was defined as a competing endogenous RNA by sponging miR-365a-3p, thus elevating phosphoglycerate kinase 1 (PGK1) expression by directly targeting its 3ʹUTR to increase aerobic glycolysis.ConclusionHCG18 promoted OS cell proliferation via enhancing aerobic glycolysis by regulating the miR-365a-3p/PGK1 axis. Therefore, HCG18 may be a potential target for OS treatment.

Highlights

Osteosarcoma (OS) is a common primary bone malignancy
We demonstrated that long noncoding RNAs (lncRNAs) HCG18 was upregulated in OS cell lines and elevated OS proliferation by increasing aerobic glycolysis
HCG18 knockdown inhibited OS proliferation in vitro To determine whether lncRNA HCG18 plays a critical role in OS progression, we first detected the expression of HCG18 in human OS cell lines (MG63, MNNG-HOS, U-2OS, and 143B) and a human osteoblast cell line

Summary

Introduction

Osteosarcoma (OS) is a common primary bone malignancy. Long noncoding RNA HCG18 is known to play an important role in a variety of cancers. Results: HCG18 expression was increased in OS cell lines. HCG18 was defined as a competing endogenous RNA by sponging miR-365a-3p, elevating phosphoglycerate kinase 1 (PGK1) expression by directly targeting its 3ʹUTR to increase aerobic glycolysis. Conclusion: HCG18 promoted OS cell proliferation via enhancing aerobic glycolysis by regulating the miR-365a-3p/PGK1 axis. Osteosarcoma (OS) is a common primary bone tumor and originates from interstitial tissue [1]. It accounts for approximately 3%-5% of malignant tumors and is common in those aged under 20 years [2]. It is essential to investigate the molecular mechanisms underlying OS development and to find new therapeutic targets for its treatment. Among ncRNAs, long noncoding RNAs (lncRNAs) are transcripts of greater than 200 bp, with limited or no protein

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Conclusion