LncRNA GATA6-AS inhibits cancer cell proliferation and promotes cancer cell apoptosis in cervical cancer by down-regulating miR-205

Jun Chen,Huzhong Zheng,Xiaoying Zhao

doi:10.1186/s12905-020-01082-7

Abstract

BackgroundDysregulated endothelial cell growth is involved in many types of human cancer, including cervical cancer. LncRNA GATA6-AS was reported to regulate endothelial cell growth, suggesting it might involve in cervical cancer. Our study was carried out to explore the involvement of GATA6-AS in cervical squamous cell carcinoma (CSCC), a subtype of cervical cancer.MethodsTo explore the expression of GATA6-AS, RT-qPCR was performed to detect GATA6-AS in plasma of 65 CSCC patients and 58 healthy females. To detect the expression of GATA6-AS, total RNAs were extracted.ResultsWe found that plasma GATA6-AS expression was down-regulated in CSCC patients than that in healthy females, and HPV infection did not significantly affect the plasma expression of GATA6-AS. Moreover, we found that plasma GATA6-AS showed diagnostic values for CSCC by performing ROC curve analysis. The expression of miR-205 in plasma was also found to be up-regulated in CSCC patients than that in healthy females and inversely correlated with the expression of GATA6-AS in CSCC patients. Furthermore, over-expression of miR-205 did not significantly affect the expression of GATA6-AS in CSCC cells, while over-expression of GATA6-AS down-regulated miR-205 expression. In addition, GATA6-AS over-expression inhibited CSCC cell proliferation and promoted CSCC cell apoptosis, while miR-205 over-expression played opposite roles and attenuated the effects of GATA6-AS over-expression on CSCC cells.ConclusionTaken together, these results suggest that GATA6-AS may inhibit cell proliferation and promote cell apoptosis in CSCC by down-regulating miR-205.

Highlights

Dysregulated endothelial cell growth is involved in many types of human cancer, including cervical cancer
It has been reported that GATA6-AS can regulate endothelial cell growth, which is known to be involve in the development of different types of human diseases, such as cervical cancer, by epigenetically regulating gene expression [12]
Plasma GATA6-AS expression was down-regulated in cervical squamous cell carcinoma (CSCC) and not affected by human papillomaviruses (HPV) infection To explore the expression of GATA6-AS, RT-qPCR was performed to detect GATA6-AS in plasma of 65 CSCC patients and 58 healthy females

Summary

Introduction

Dysregulated endothelial cell growth is involved in many types of human cancer, including cervical cancer. There is accumulative evidence showing that different types of ncRNAs, such as long (> 200 nt) ncRNAs (lncRNAs), involve in diverse physiological and pathological processes, such as tumorigenesis [9, 10] It has been well-established that certain lncRNAs in cancers, such as cervical cancer, regulate the expression of oncogenic and/or tumor suppressive genes to promote or inhibit cancer development [9, 10]. It has been reported that GATA6-AS can regulate endothelial cell growth, which is known to be involve in the development of different types of human diseases, such as cervical cancer, by epigenetically regulating gene expression [12]. Our study was carried out to investigate the involvement of GATA6-AS in cervical squamous cell carcinoma (CSCC), a major subtype of cervical cancers, and to explore its possible interaction with miR-205

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Discussion

Conclusion