Abstract
Objective: Long noncoding RNA growth arrest-specific transcript 5 (lncRNA GAS5) is involved in various kinds of cancer. However, the role of lncGAS5 in the development of ovarian cancer remains unclear. In the present study, we explored the cellular mechanism and clinical value of lncRNA GAS5 in ovarian cancer.Methods: Quantitative real-time PCR was used to detect mRNA level of lncRNA GAS5 in 20 ovarian cancer tissues. The effect of lncRNA GAS5 on cell proliferation was performed using CCK-8 assay. Cell apoptosis was evaluated by flow cytometry. Western blotting was used to detect the protein level of lncRNA GAS5 potential target. Standard sandwich ELISA was used to quantify the level of inflammatory cytokines. The cells with stable expression of lncRNA GAS5 were injected into nude mice to study the effect of lncRNA GAS5 on tumorigenesis in vivo.Results: The expression of lncRNA GAS5 was significantly decreased in ovarian cancer tissues. Decrease in lncRNA GAS5 expression resulted in increased cell proliferation and colony formation and reduced ovarian cancer cell apoptosis. In contrast, exogenous overexpression of lncRNA GAS5 in ovarian cancer cells inhibited proliferation, colony formation, and apoptosis in ovarian cancer cells. In addition, the role of lncRNA GAS5 in ovarian cancer was associated with inflammasome formation and pyroptosis.Conclusion: These results suggested that lncRNA GAS5 acts as tumor suppressor and could be used as a potential treatment target for diagnosis and therapy of ovarian cancer.
Highlights
Ovarian cancer is the fifth most common cancer type in females worldwide, and the second most frequent malignant tumor in females, which accounts for almost 3% of cancer in females [1]
Both of the excised ovarian cancer tissues and matched adjacent normal tissues were collected for the analysis. quantitative real-time PCR (qRT-PCR) was used to examine lncGAS5 expression in the included samples
Our results suggested that pyroptosis in these long noncoding RNAs (lncRNAs) growth arrest-specific transcript 5 (GAS5) overexpressed cells was induced in a time-dependent manner
Summary
Ovarian cancer is the fifth most common cancer type in females worldwide, and the second most frequent malignant tumor in females, which accounts for almost 3% of cancer in females [1]. Studies have suggested that over 98% of the human genome contains noncoding proteins sequences, with a majority of them being transcribed into nonprotein-coding RNA transcripts [3]. The size of these transcripts differs from very small RNAs, defined as microRNAs (miRNAs), which contains 20–25 base pairs, to long noncoding RNAs (lncRNAs) that contain over 100 kb [4]. LncRNAs are capable of interacting with different types of biomolecules, such as DNA, RNA and proteins, and play vital roles in gene expression regulation at transcriptional, post-transcriptional, and epigenetic levels [5]. LncRNAs play a critical role in tumorigenesis and tumor progression [6]. LncRNAs can be divided into three types: License 4.0 (CC BY)
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