Abstract
BackgroundLong non-coding RNA growth arrest specific 5 (GAS5) is a regulator in non-small cell lung cancer (NSCLC) progression. Nonetheless, the mechanism by which GAS5 exerts its biological function in NSCLC cells remains unclear.MethodsGAS5, miR-221-3p relative expression levels in NSCLC tissues and cells were examined by qPCR. After gain-of-function and loss-of-function models were established, the viability of H1299 and A549 cells were examined by CCK-8 and EdU assays. Cell migration and invasion were examined by the Transwell experiment. The binding sequence of GAS5 for miR-221-3p was confirmed by the dual-luciferase reporter gene experiment. The regulatory function of GAS5 and miR-221-3p on IRF2 was investigated by Western blot.ResultsGAS5 expression was down-modulated in NSCLC tissues and cell lines. GAS5 overexpression restrained the proliferation, migration and invasion of NSCLC cells, while miR-221-3p, which was targeted and negatively modulated by GAS5, worked oppositely. Restoration of miR-221-3p markedly reversed the effects of GAS5 on NSCLC cells. Additionally, GAS5 increased IRF2 expression in NSCLC cells by repressing miR-221-3p.ConclusionsGAS5 blocks the progression of NSCLC partly via increasing IRF2 expression level via repressing miR-221-3p.
Highlights
Lung cancer is the most common cancer, with about 80 % of cases being non-small cell lung cancer (NSCLC) [1]
growth arrest specific 5 (GAS5) expression was abnormally decreased in NSCLC tissues and cell lines To detect GAS5 expression in NSCLC, firstly, Quantitative real-time polymerase chain reaction (qPCR) was performed
The GAS5 overexpression plasmid was transfected into H1299 and the GAS5 shRNA was transfected into A549, respectively, to construct the gain-of-function and lossof-function models, and the transfection efficiency was verified using qPCR (Fig. 1 c)
Summary
Lung cancer is the most common cancer, with about 80 % of cases being non-small cell lung cancer (NSCLC) [1]. There is an urgent need for Reportedly, long non-coding RNAs (lncRNAs) regulate the development of diverse cancers. It is reported that lncRNA growth arrest specific 5 (GAS5) is a tumor suppressor in multiple cancers including NSCLC [4, 5]. The specific molecular mechanism by which GAS5 suppresses NSCLC progression has not been fully elucidated. MicroRNAs (miRNAs), are vital regulators in diverse cancers. Accumulating studies indicate that miR-221-3p is a cancer-related miRNA. Long non-coding RNA growth arrest specific 5 (GAS5) is a regulator in non-small cell lung cancer (NSCLC) progression. The mechanism by which GAS5 exerts its biological function in NSCLC cells remains unclear
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