Abstract
BackgroundLong noncoding RNAs (lncRNAs) have been reported to be associated with the proliferation of several cancer cells. The aim of this study was to investigate the role of FLVCR1-AS1 in ovarian serous cancer (OSC).MethodsFLVCR1-AS1 expression was determined in human OSC tissues, serums and cell lines. The role of FLVCR1-AS1 knockdown or overexpression on OSC cell growth, migration, invasion, apoptosis and epithelial to mesenchymal transition (EMT) were evaluated in vitro using CCK8, colony formation assay, wound healing assay, transwell assay and western blot assay. Besides, luciferase reporter assays were performed to identify interactions among FLVCR1-AS1 and its target genes. Moreover, the in vivo effects were investigated using immunocompromised NSG female mice.ResultsIn this study, FLVCR1-AS1 expression was upregulated in OSC tissues, serums, and cells. Knockdown FLVCR1-AS1 decreased cell growth, migration, invasion, and EMT, as well as increased apoptosis in OSC cells, whereas, overexpression of FLVCR1-AS1 increased cell proliferation, migration, invasion, and EMT, and decreased apoptosis of OSC cells. Besides, FLVCR1-AS1 directly bound to miR-513 and downregulated its expression. Moreover, FLVCR1-AS1 reversed the effect of miR-513 on the OSC cell growth, which might be associated with the role of YAP1. Furthermore, in terms of mechanism, FLVCR1-AS1 promoted EMT in OSC cells. Finally, mice models further confirmed that knockdown FLVCR1-AS1 distinctly suppressed cell growth and EMT in vivo.ConclusionTaken together, FLVCR1-AS1 mediated miR-513/YAP1 signaling to promote cell progression, migration, invasion and EMT process in OSC cells.
Highlights
Long noncoding RNAs have been reported to be associated with the proliferation of several cancer cells
FLVCR1-AS1 expression is upregulated in ovarian serous cancer (OSC) tissues, serums, and cell lines and predicted poor prognosis Results showed that FLVCR1-AS1 expression was upregulated in OSC tissues compared with adjacent non-tumor tissues (Fig. 1a, P < 0.01)
FLVCR1-AS1 expression was increased in the serum of OSC patients compared with health controls (Fig. 1b, P < 0.01), and a positive association was observed between mRNA levels of FLVCR1-AS1 in OSC serum and tissues (Fig. 1c; r2 = 0.7460, P < 0.0001)
Summary
Long noncoding RNAs (lncRNAs) have been reported to be associated with the proliferation of several cancer cells. The aim of this study was to investigate the role of FLVCR1-AS1 in ovarian serous cancer (OSC). Long non-coding RNAs (lncRNAs) play a pivotal role in cancer development, especially in cancer occurrence and metastasis, cell proliferation and apoptosis [5,6,7]. In OSC, dysfunction of lncRNAs was reported to be associated with cell progression and metastasis. Previous study showed that lncRNA DQ786243 was upregulated in OSC cells, and knockdown of DQ786243 inhibited cell progression of via regulating miR-506 [8]. LncRNA MLK7-AS1 promoted migration of OSC cells via miR-375/YAP1 axis [9].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
