LncRNA FGD5-AS1 Regulates Bone Marrow Stem Cell Proliferation and Apoptosis by Affecting miR-296-5p/STAT3 Axis in Steroid-Induced Osteonecrosis of the Femoral Head.

Yadi Wu,Gang Zhang,Yong Gao,Zhiqiu Zhao,Lun Fang,Lu Zhou,M A Bhagyaveni

doi:10.1155/2022/9364467

Abstract

Background Osteonecrosis of the femoral head (ONFH) is a common hip joint disease, which is more harmful and seriously affects the lives of patients. This study aims to clarify the regulatory mechanism of lncRNA FGD5-AS1 in ONFH. Methods The expression of the protein and mRNA was detected by RT-qPCR and Western blot assay. The regulatory mechanism of lncRNA FGD5-AS1 was detected by the dual-luciferase reporter assay, CCK-8 assay, and flow cytometry assay. Results Dex can inhibit cell proliferation and differentiation and induce apoptosis in hBMSCs in a dose-dependent manner. Overexpression of lncRNA FGD5-AS1 promoted cell proliferation and restrained apoptosis in Dex-treated hBMSCs. In addition, lncRNA FGD5-AS1 acts as a sponge for miR-296-5p. Also, miR-296-5p directly targets STAT3. More importantly, miR-296-5p and STAT3 can affect the function of lncRNA FGD5-AS1 in Dex-treated hBMSCs. Conclusion lncRNA FGD5-AS1 promotes cell proliferation and inhibits apoptosis in steroid-induced ONFH through acting as a sponge for miR-296-5p and upregulation of STAT3.

Highlights

Osteonecrosis of the femoral head (ONFH) is a disease of femoral head structural changes, femoral head collapse, and joint dysfunction caused by interruption or damage to the blood supply of the femoral head [1]
The effects of Dex (0, 10−6, 10−7, and 10−8 M) on cell proliferation, apoptosis, and OPG/RANK/RANKL pathway were investigated in human bone marrow-derived mesenchymal stem cells (hBMSCs)
The inhibitory effect of Dex on cell proliferation was increased as the concentration increases (P < 0.05, Figure 1(a)). e western blot assay and RT-qPCR showed the decreased expression of OPG and increased expression of RANK and RANKL in hBMSCs treated with different concentrations of Dex (P < 0.05, Figure 1(b))

Summary

Introduction

Osteonecrosis of the femoral head (ONFH) is a disease of femoral head structural changes, femoral head collapse, and joint dysfunction caused by interruption or damage to the blood supply of the femoral head [1]. Nontraumatic ONFH is a refractory disease in the field of orthopedics with a very high incidence [2]. It is caused by nontraumatic factors such as glucocorticoids or excessive alcohol consumption [3]. Osteonecrosis of the femoral head (ONFH) is a common hip joint disease, which is more harmful and seriously affects the lives of patients. Overexpression of lncRNA FGD5-AS1 promoted cell proliferation and restrained apoptosis in Dex-treated hBMSCs. In addition, lncRNA FGD5-AS1 acts as a sponge for miR-296-5p. MiR-2965p and STAT3 can affect the function of lncRNA FGD5-AS1 in Dex-treated hBMSCs. Conclusion. LncRNA FGD5-AS1 promotes cell proliferation and inhibits apoptosis in steroid-induced ONFH through acting as a sponge for miR-296-5p and upregulation of STAT3 Conclusion. lncRNA FGD5-AS1 promotes cell proliferation and inhibits apoptosis in steroid-induced ONFH through acting as a sponge for miR-296-5p and upregulation of STAT3

Methods

Results

Conclusion