LncRNA DSCAM-AS1 Negatively Interacts with miR-124 to Promote Hepatocellular Carcinoma Proliferation.

Abstract

It is known that the development of resistance of breast cancer cells to chemotherapy is at least partially mediated by the upregulation of long non-coding RNA DSCAM antisense RNA 1 (DSCAM-AS1). Therefore, DSCAM-AS1 may play a role in cancer biology. The differential expression of DSCAM-AS1 and miR-124 in hepatocellular carcinoma (HCC) was analyzed by quantitative polymerase chain reaction. One-way analysis of variance (ANOVA) Tukey test was used to analyze the differences in gene expression and cell proliferation in HCC cell lines. Expression levels of DSCAM-AS1 and miR-124 were compared between HCC and paired non-tumor tissues by paired t-test. The effects of transfections on SNU-449 and SNU-398 cell proliferation were analyzed by CCK-8 assay. DSCAM-AS1 was upregulated, while miR-124 was downregulated in HCC tissues. DSCAM-AS1 and miR-124 were inversely correlated across HCC tissues but not normal tissues. DSCAM-AS1 level increased, while miR-124 level decreased along with the clinical stage increasing. miR-124 overexpression increased DSCAM-AS1 level while DSCAM-AS1 overexpression decreased miR-124 level. In addition, miR-124 overexpression decreased HCC cell proliferation, while DSCAM-AS1 increased HCC cell proliferation. Moreover, DSCAM-AS1 overexpression reduced the effects of miR-124 overexpression. DSCAM-AS1 interacts with miR-124 to promote HCC proliferation.