Abstract
BackgroundLong non-coding RNAs (lncRNAs) have different functions in cells. They work as signals, decoys, guides, and scaffolds. Altered lncRNA levels can affect the expression of gene products. There are seldom studies on the role of lncRNAs in inflammatory bowel disease (IBD).ResultsQuantitative RT-PCR showed that DQ786243 was significantly overexpressed in clinical active CD patients compared with clinical inactive CD patients (P = 0.0118) or healthy controls (P = 0.002). CREB was also more highly expressed in active CD than in inactive CD (P = 0.0034) or controls (P = 0.0241). Foxp3 was interestingly lower in inactive CD than in active CD (P = 0.0317) or controls (P = 0.0103), but there were no apparent differences between active CD and controls. CRP was well correlated with DQ786243 (r = 0.489, P = 0.034), CREB (r = 0.500, P = 0.029) and Foxp3 (r = 0.546, P = 0.016). At 48 hours after DQ786243 transfection, qRT-PCR showed both CREB (P = 0.017) and Foxp3 (P = 0.046) had an increased mRNA expression in Jurkat cells. Western blot showed the same pattern. After DQ786243 transfection, CREB phosphorylation ratio (p-CREB/t-CREB) was increased (P = 0.0043).ConclusionDQ786243 can be related with severity of CD. It can affect the expression of CREB and Foxp3 through which regulates the function of Treg. CREB itself seems not the mediator of DQ786243 to up-regulate Foxp3. The phosphorylation of CREB might play a more important role in the process.
Highlights
Long non-coding RNAs have different functions in cells
The relationship of RNA levels and clinical inflammation serological markers It showed that C-reactive protein (CRP) was well correlated with DQ786243 (r = 0.489, P = 0.034), cAMP response element binding protein (CREB) (r = 0.500, P = 0.029) and forkhead box P3 (Foxp3) (r = 0.546, P = 0.016) (See Figure 2A, B and C)
The expression of DQ786243 was correlated with CREB (r = 0.552, P = 0.002) and Foxp3 (r = 0.435, P = 0.021) at a significant level, but there was no significant correlation between CREB and Foxp3 (See Figure 2G, H and I)
Summary
Long non-coding RNAs (lncRNAs) have different functions in cells. Altered lncRNA levels can affect the expression of gene products. Long non-coding RNAs (lncRNAs) are non-protein coding transcripts over 200 bases in length. In functional genomics, they belong to a new type of regulatory genes. LncRNAs regulate protein coding gene at the level of chromatin remodeling, transcriptional control and post-transcriptional processing [7]. They work as signals, decoys, guides, and scaffolds [8] They may “sponge” mi-RNAs to regulate the gene expression [9]. Many researches showed altered lncRNA levels could affect the expression of gene products. LncRNAs play critical roles in cancer biology [12,13]. There are seldom studies on the role of lncRNAs in inflammatory bowel disease (IBD)
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