LncRNA DLEU1 is overexpressed in premature ovarian failure and sponges miR-146b-5p to increase granulosa cell apoptosis

Caihong Zheng,Zhihong Qin,Xiaoqian Zhang,Shiwei Liu,Yubao Song

doi:10.1186/s13048-021-00905-x

Abstract

BackgroundmiR-146b-5p has been reported to participate in premature ovarian failure (POF) in mice. However, its role in POF patients is unclear. We predicted that miR-146b-5p might interact with lncRNA DLEU1, a crucial player in ovarian cancer. We then explored the interaction between DLEU1 and miR-146b-5p.MethodsExpression of DLEU1 and miR-146b-5p in POF and control ovary tissues was determined by RT-qPCR. The subcellular location of DLEU1 in human KGN cells was analyzed using subcellular fractionation assays. The direct interaction between DLEU1 and miR-146b-5p was analyzed using RNA pull-down assays. The role of DLEU1 in miR-146a expression was analyzed using overexpression assay. Cell proliferation was analyzed using cell apoptosis assay.ResultsIncreased DLEU1 expression and decreased miR-146b-5p expression were observed in POF. DLEU1 directly interacted with MiR-146b-5p and was expressed in both nuclear and cytoplasm samples of KGN cells. In KGN cells, DLEU1 and miR-146b-5p failed to regulate the expression of each other. However, DLEU1 promoted cell apoptosis and reduced the inhibitory effects of miR-146b-5p on cell apoptosis.ConclusionsDLEU1 is overexpressed in POF and sponges miR-146b-5p to increase KGN cell apoptosis.

Highlights

Premature ovarian failure (POF), known as primary ovarian insufficiency, is a common cause of infertility and is characterized by hypoestrogenism, elevated gonadotropins, and amenorrhea [1, 2]
Exploration of DLEU1 and miR‐146b‐5p expression in premature ovarian failure (POF) Total RNAs were isolated from Granulosa cells (GCs) samples from both POF patients (n = 49) and controls (n = 49) and subjected to RTs and qPCRs to explore the differential expression of DLEU1 and miR-146b-5p in POF
The results showed that DLEU1 expression was increased (Fig. 1A, p < 0.01), and miR-146b-5p expression was decreased (Fig. 1B, p < 0.01) in POF

Summary

Introduction

Premature ovarian failure (POF), known as primary ovarian insufficiency, is a common cause of infertility and is characterized by hypoestrogenism, elevated gonadotropins, and amenorrhea [1, 2]. With the development of POF, menopause will occur, leading to ovarian primordial follicle pool premature depletion [4, 5]. At. Understanding the molecular mechanism of POF may provide novel insights into the development of novel antiPOF strategies [9,10,11]. More targets, especially those with high safety and efficiency, are still needed [9,10,11,12]. MiR-146b-5p has been reported to participate in premature ovarian failure (POF) in mice. We predicted that miR-146b-5p might interact with lncRNA DLEU1, a crucial player in ovarian cancer. We explored the interaction between DLEU1 and miR-146b-5p

Methods

Results

Conclusion