LncRNA DANCR functions as a competing endogenous RNA to regulate RAB1A expression by sponging miR-634 in glioma.

Dawei Xu,Pengju Ma,Yi Zhang,Guojun Gao,Jian Yu,Guangjian Lu

doi:10.1042/bsr20171664

Abstract

Long noncoding RNA (lncRNA) differentiation antagonizing nonprotein coding RNA (DANCR) plays important regulatory roles in many solid tumors. However, the effect of DANCR in glioma progression and underlying molecular mechanisms were not entirely explored. In the present study, we determined the expression of DANCR in glioma tissues and cell lines using qRT-PCR and further defined the biological functions. Furthermore, we used luciferase reporter assay, Western blot, and RNA immunoprecipitation (RIP) to explore the underlying mechanism. Our results showed that DANCR was significantly up-regulated in glioma tissues and cell lines (U251, U118, LN229, and U87MG). High DANCR expression was correlated with advanced tumor grade. Inhibition of DANCR suppressed the glioma cells proliferation and induced cells arrested in the G0/G1 phase. In addition, we verified that DANCR could directly interact with miR-634 in glioma cells and this interaction resulted in the inhibition of downstream of RAB1A expression. The present study demonstrated that DANCR/miR-634/RAB1A axis plays crucial roles in the progression of glioma, and DANCR might potentially serve as a therapeutic target for the treatment of glioma patients.

Highlights

Glioma is the most lethal and common type of primary malignant brain tumor in the central nervous system of adults with an annual incidence of five cases per 100,000 people [1]
To identify the role of Differentiation antagonizing nonprotein coding RNA (DANCR) in glioma progression, we analyzed the expression of DANCR in 47 glioma tissue samples and 14 normal brain (NB) tissue samples using qRT-PCR
Many studies demonstrated that DANCR might act as an oncogenic Long noncoding RNA (lncRNA) in tumor progression

Summary

Introduction

Glioma is the most lethal and common type of primary malignant brain tumor in the central nervous system of adults with an annual incidence of five cases per 100,000 people [1]. Long noncoding RNA (lncRNA) is nonprotein coding transcripts more than 200 nucleotides (nt) in length [4]. LncRNA has been confirmed to act as key molecules in the progression of various human tumors [5,6]. Differentiation antagonizing nonprotein coding RNA (DANCR) is located on human chromosome 4, with the closest adjacent annotated genes USP46 and ERVMER34-1 [7]. Recent studies indicated that DANCR was up-regulated in a large variety of cancers and thereby of high diagnostic value for great clinical value for cancer therapy. The expression and the underlying roles of DANCR in glioma are still unclear

Methods

Results

Conclusion